J
Javier Gil-Humanes
Researcher at University of Minnesota
Publications - 30
Citations - 2789
Javier Gil-Humanes is an academic researcher from University of Minnesota. The author has contributed to research in topics: Gluten & Gliadin. The author has an hindex of 22, co-authored 30 publications receiving 2223 citations. Previous affiliations of Javier Gil-Humanes include Spanish National Research Council.
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Journal ArticleDOI
A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants
Tomas Cermak,Shaun J. Curtin,Javier Gil-Humanes,Radim Cegan,Thomas J. Y. Kono,Eva Konečná,Joseph J. Belanto,Colby G. Starker,Jade W. Mathre,Rebecca L. Greenstein,Daniel F. Voytas +10 more
TL;DR: An integrated reagent toolkit and streamlined protocols work across diverse plant species to enable sophisticated genome edits and it is demonstrated that Cas9 nickases induce gene targeting at frequencies comparable to native Cas9 when they are delivered on geminivirus replicons.
Journal ArticleDOI
DNA Replicons for Plant Genome Engineering
TL;DR: The feasibility of using geminivirus replicons to generate plants with a desired DNA sequence modification is demonstrated and the results advocate the use of replicons for plant genome engineering.
Journal ArticleDOI
Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9.
Susana Sánchez-León,Javier Gil-Humanes,Carmen V. Ozuna,María J. Giménez,Carolina Sousa,Daniel F. Voytas,Francisco Barro +6 more
TL;DR: The low‐gluten, transgene‐free wheat lines described here could be used to produce low-gluten foodstuff and serve as source material to introgress this trait into elite wheat varieties.
Journal ArticleDOI
High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9.
Javier Gil-Humanes,Yanpeng Wang,Zhen Liang,Qiwei Shan,Carmen V. Ozuna,Susana Sánchez-León,Nicholas J. Baltes,Colby G. Starker,Francisco Barro,Caixia Gao,Daniel F. Voytas +10 more
TL;DR: High frequencies of GT using WDV-based DNA replicons will make it possible to edit complex cereal genomes without the need to integrate GT reagents into the genome.
Journal ArticleDOI
Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference
Javier Gil-Humanes,Fernando Pistón,Stig Tollefsen,Stig Tollefsen,Ludvig M. Sollid,Francisco Barro +5 more
TL;DR: This work shows that the down-regulation of gliadins by RNAi can be used to obtain wheat lines with very low levels of toxicity for CD patients.