J
Johan Ledin
Researcher at Science for Life Laboratory
Publications - 28
Citations - 2679
Johan Ledin is an academic researcher from Science for Life Laboratory. The author has contributed to research in topics: Heparan sulfate & Zebrafish. The author has an hindex of 16, co-authored 27 publications receiving 2378 citations. Previous affiliations of Johan Ledin include Swedish University of Agricultural Sciences & Uppsala University.
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Journal ArticleDOI
Abnormal mast cells in mice deficient in a heparin-synthesizing enzyme
Erik Forsberg,Gunnar Pejler,Maria Ringvall,Carolina Lunderius,Bianca R. Tomasini-Johansson,Marion Kusche-Gullberg,Inger Eriksson,Johan Ledin,Lars Hellman,Lena Kjellén +9 more
TL;DR: The results indicate that one site of physiological action for heparin could be inside connective-tissue-type mast cells, where its absence results in severe defects in the secretory granules.
Journal ArticleDOI
High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9
Gaurav K. Varshney,Wuhong Pei,Matthew C. LaFave,Jennifer Idol,Lisha Xu,Viviana E Gallardo,Blake Carrington,Kevin Bishop,MaryPat Jones,Mingyu Li,Ursula Harper,Sunny C. Huang,Anupam Prakash,Wenbiao Chen,Raman Sood,Johan Ledin,Shawn M. Burgess +16 more
TL;DR: A cloning-free single-guide RNA (sgRNA) synthesis, coupled with streamlined mutant identification methods utilizing fluorescent PCR and multiplexed, high-throughput sequencing is described, which shows that efficiency of CRISPR/Cas9 is sixfold more efficient than other techniques.
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Heparan Sulfate Structure in Mice with Genetically Modified Heparan Sulfate Production
Johan Ledin,William D. Staatz,Jin-Ping Li,Martin Götte,Scott B. Selleck,Lena Kjellén,Dorothe Spillmann +6 more
TL;DR: Using a high throughput heparan sulfate (HS) isolation and characterization protocol, HS structure in several tissues from mice/mouse embryos deficient in HS biosynthesis enzymes and in mice lacking syndecan-1 are analyzed.
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Defective heparan sulfate biosynthesis and neonatal lethality in mice lacking N-deacetylase/N-sulfotransferase-1
Maria Ringvall,Johan Ledin,Katarina Holmborn,Toin H. van Kuppevelt,Fredrik Ellin,Inger Eriksson,Anne-Mari Olofsson,Lena Kjellén,Erik Forsberg +8 more
TL;DR: It is reported that targeted gene disruption of NDST-1 in the mouse results in a structural alteration of heparan sulfate in most basement membranes as revealed by immunohistochemical staining of fetal tissue sections using antibodies raised against heparin sulfate.
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Defective N-sulfation of heparan sulfate proteoglycans limits PDGF-BB binding and pericyte recruitment in vascular development
Alexandra Abramsson,Sindhulakshmi Kurup,Marta Busse,Shuhei Yamada,Per Lindblom,Edith Schallmeiner,Denise Stenzel,Dominique Sauvaget,Johan Ledin,Maria Ringvall,Ulf Landegren,Lena Kjellén,Göran Bondjers,Jin-Ping Li,Ulf Lindahl,Dorothe Spillmann,Christer Betsholtz,Holger Gerhardt +17 more
TL;DR: It is concluded that pericyte recruitment requires HS with sufficiently extended and appropriately spaced N-sulfated domains to retain PDGF-BB and activate PDGF receptor beta (PDGFRbeta) signaling, whereas the detailed sequence of monosaccharide and sulfate residues does not appear to be important for this interaction.