J
John Manzi
Researcher at PSL Research University
Publications - 36
Citations - 1853
John Manzi is an academic researcher from PSL Research University. The author has contributed to research in topics: Membrane & Membrane curvature. The author has an hindex of 17, co-authored 32 publications receiving 1406 citations. Previous affiliations of John Manzi include Centre national de la recherche scientifique & Curie Institute.
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Journal ArticleDOI
Nature of curvature coupling of amphiphysin with membranes depends on its bound density
Benoit Sorre,Andrew Callan-Jones,John Manzi,Bruno Goud,Jacques Prost,Patricia Bassereau,Aurélien Roux +6 more
TL;DR: The approach shows that the strength of curvature sensing and mechanical effects on the tube depends on the protein density, and the distribution of proteins and the mechanical effects induced are described by a model based on spontaneous curvature induction.
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Nanoplasmonic Sensor Detects Preferential Binding of IRSp53 to Negative Membrane Curvature.
Gustav Emilsson,Evelyn Röder,Bita Malekian,Kunli Xiong,John Manzi,Feng-Ching Tsai,Nam-Joon Cho,Marta Bally,Andreas B. Dahlin +8 more
TL;DR: This work shows the first example of analyzing preferential binding of an average-sized and biologically important protein to negative membrane curvature in a label-free manner and in real-time, illustrating a unique application for nanoplasmonic sensors.
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IRSp53 senses negative membrane curvature and phase separates along membrane tubules
Coline Prévost,Hongxia Zhao,John Manzi,John Manzi,John Manzi,Emmanuel Lemichez,Pekka Lappalainen,Andrew Callan-Jones,Patricia Bassereau,Patricia Bassereau,Patricia Bassereau +10 more
TL;DR: An original assay in which proteins are encapsulated in giant unilamellar vesicles connected to membrane nanotubes is developed, demonstrating that I-BAR dimers sense negative membrane curvature and allows constriction of weakly curved membranes coupled to local protein enrichment at biologically relevant conditions.
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A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats.
TL;DR: The results show that membrane tension controls clathrin-mediated budding by varying the membrane budding energy, and the theoretical framework is used to estimate the polymerization energy from these data.
Journal ArticleDOI
Membrane Shape at the Edge of the Dynamin Helix Sets Location and Duration of the Fission Reaction
Sandrine Morlot,Valentina Galli,Marius Klein,Nicolas Chiaruttini,John Manzi,Frédéric Humbert,Luis Dinis,Martin Lenz,Martin Lenz,Giovanni Cappello,Aurélien Roux +10 more
TL;DR: It is shown that fission occurs at the interface between the dynamin coat and the uncoated membrane, and that the mechanical energy spent on dynamin constriction can reduce the energy barrier for fission sufficiently to promote spontaneous fission.