J
Joke A. Bouwstra
Researcher at Leiden University
Publications - 375
Citations - 23433
Joke A. Bouwstra is an academic researcher from Leiden University. The author has contributed to research in topics: Stratum corneum & Corneocyte. The author has an hindex of 78, co-authored 368 publications receiving 20996 citations. Previous affiliations of Joke A. Bouwstra include VU University Amsterdam & University of Illinois at Urbana–Champaign.
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Journal ArticleDOI
Layer-by-Layer Assembly of Inactivated Poliovirus and N-Trimethyl Chitosan on pH-Sensitive Microneedles for Dermal Vaccination
Koen van der Maaden,Emine Sekerdag,Pim Schipper,Gideon F.A. Kersten,Wim Jiskoot,Joke A. Bouwstra +5 more
TL;DR: Topical administration of pH-sensitive microneedles coated with polyelectrolyte multinanolayers of antigens and oppositely charged polymers may be a useful approach for micronedle-based vaccination.
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Microneedle-Based Transcutaneous Immunisation in Mice with N-Trimethyl Chitosan Adjuvanted Diphtheria Toxoid Formulations
TL;DR: TMC has an adjuvant function in transcutaneous immunisation with microneedles, but only if applied in a solution.
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Novel Hollow Microneedle Technology for Depth-Controlled Microinjection-Mediated Dermal Vaccination: A Study with Polio Vaccine in Rats
Koen van der Maaden,Sebastiaan Johannes Trietsch,Heleen Kraan,Eleni Maria Varypataki,Stefan Romeijn,Raphaël Zwier,Heiko J. van der Linden,Gideon F.A. Kersten,Thomas Hankemeier,Wim Jiskoot,Joke A. Bouwstra +10 more
TL;DR: A hollow microneedle technology for dermal vaccination that enables fundamental research on factors, such as insertion depth and volume, and insertion angle, on the immune response is successfully developed.
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Focus on skin as a possible port of entry for solid nanoparticles and the toxicological impact.
TL;DR: A review of data published on NP skin penetration, toxicological issues and on physicochemical NP characterisation focuses on skin as a route of exposure for NPs and the toxicological impact in skin with special attention to physicochemical properties of NPsand skin.
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Culture of reconstructed epidermis in a defined medium at 33°C shows a delayed epidermal maturation, prolonged lifespan and improved stratum corneum
TL;DR: Keratin 6 expression was not directly linked to hyperproliferation but to deregulated terminal differentiation, and the epidermal lipid profile was better in cultures grown in medium I; the relative amounts of ceramides, free fatty acids and cholesterol being comparable to native epidermis.