J
Josip Blonder
Researcher at Science Applications International Corporation
Publications - 62
Citations - 3230
Josip Blonder is an academic researcher from Science Applications International Corporation. The author has contributed to research in topics: Proteomics & Membrane protein. The author has an hindex of 33, co-authored 62 publications receiving 3016 citations. Previous affiliations of Josip Blonder include Environmental Molecular Sciences Laboratory & National Institutes of Health.
Papers
More filters
Journal ArticleDOI
Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach
Steven A. Carr,Susan E. Abbatiello,Bradley L. Ackermann,Christoph H. Borchers,Bruno Domon,Eric W. Deutsch,Russell P. Grant,Andrew N. Hoofnagle,Ruth Hüttenhain,Ruth Hüttenhain,John M. Koomen,Daniel C. Liebler,Tao Liu,Brendan MacLean,D. R. Mani,Elizabeth Mansfield,Hendrik Neubert,Amanda G. Paulovich,Lukas Reiter,Olga Vitek,Ruedi Aebersold,Leigh Anderson,Robert Bethem,Josip Blonder,Emily S. Boja,Julianne Cook Botelho,Michael T. Boyne,Ralph A. Bradshaw,Alma L. Burlingame,Daniel W. Chan,Hasmik Keshishian,Eric Kuhn,Christopher R. Kinsinger,Jerry S.H. Lee,Jerry S.H. Lee,Sang Won Lee,Robert L. Moritz,Juan A. Oses-Prieto,Nader Rifai,James C. Ritchie,Henry Rodriguez,Pothur R. Srinivas,Raymond R. Townsend,Jennifer E. Van Eyk,Gordon Whiteley,Arun P. Wiita,Susan T. Weintraub +46 more
TL;DR: A workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays and defined three tiers of assays distinguished by their performance and extent of analytical characterization.
Journal ArticleDOI
Enrichment of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry.
Josip Blonder,Michael B. Goshe,Ronald J. Moore,Ljiljana Paša-Tolić,Christophe Masselon,Mary S. Lipton,Richard D. Smith +6 more
TL;DR: A sample preparation method combining carbonate extraction, surfactant-free organic solvent-assisted solubilization, and proteolysis was developed and demonstrated to target the membrane subproteome of Deinococcus radiodurans, validates the efficacy of the described sample preparation technique to isolate andsolubilize hydrophobic integral membrane proteins from complex protein mixtures.
Journal ArticleDOI
Identification of a Dynamic Mitochondrial Protein Complex Driving Cholesterol Import, Trafficking, and Metabolism to Steroid Hormones
Malena B. Rone,Andrew Midzak,Leeyah Issop,Georges Rammouz,Sathvika Jagannathan,Jinjiang Fan,Xiaoying Ye,Josip Blonder,Timothy D. Veenstra,Vassilios Papadopoulos +9 more
TL;DR: A bioactive, multimeric protein complex spanning the OMM and IMM unit that is responsible for the hormone-induced import, segregation, targeting, and metabolism of cholesterol is identified.
Journal ArticleDOI
A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and the human epidermal membrane proteome.
Josip Blonder,Thomas P. Conrads,Li-Rong Yu,Atsushi Terunuma,George M. Janini,Haleem J. Issaq,Jonathan C. Vogel,Timothy D. Veenstra +7 more
TL;DR: Due to its simplicity, efficiency, and absence of MS interfering compounds, this technique can be used for the characterization of other integral membrane proteins and may be concomitantly applied for the analysis of membrane protein complexes or large‐scale proteomic studies of different membrane samples.
Journal ArticleDOI
18O stable isotope labeling in MS-based proteomics.
TL;DR: Different strategies employing (16)O/(18)O are examined in the context of global comparative proteome profiling, targeted subcellular proteomics, analysis of post-translational modifications and biomarker discovery.