H
Hendrik Neubert
Researcher at Pfizer
Publications - 56
Citations - 2138
Hendrik Neubert is an academic researcher from Pfizer. The author has contributed to research in topics: Liquid chromatography–mass spectrometry & Medicine. The author has an hindex of 20, co-authored 51 publications receiving 1763 citations.
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Journal ArticleDOI
Targeted Peptide Measurements in Biology and Medicine: Best Practices for Mass Spectrometry-based Assay Development Using a Fit-for-Purpose Approach
Steven A. Carr,Susan E. Abbatiello,Bradley L. Ackermann,Christoph H. Borchers,Bruno Domon,Eric W. Deutsch,Russell P. Grant,Andrew N. Hoofnagle,Ruth Hüttenhain,Ruth Hüttenhain,John M. Koomen,Daniel C. Liebler,Tao Liu,Brendan MacLean,D. R. Mani,Elizabeth Mansfield,Hendrik Neubert,Amanda G. Paulovich,Lukas Reiter,Olga Vitek,Ruedi Aebersold,Leigh Anderson,Robert Bethem,Josip Blonder,Emily S. Boja,Julianne Cook Botelho,Michael T. Boyne,Ralph A. Bradshaw,Alma L. Burlingame,Daniel W. Chan,Hasmik Keshishian,Eric Kuhn,Christopher R. Kinsinger,Jerry S.H. Lee,Jerry S.H. Lee,Sang Won Lee,Robert L. Moritz,Juan A. Oses-Prieto,Nader Rifai,James C. Ritchie,Henry Rodriguez,Pothur R. Srinivas,Raymond R. Townsend,Jennifer E. Van Eyk,Gordon Whiteley,Arun P. Wiita,Susan T. Weintraub +46 more
TL;DR: A workshop was held at the National Institutes of Health with representatives from the multiple communities developing and employing targeted MS assays and defined three tiers of assays distinguished by their performance and extent of analytical characterization.
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Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays
Andrew N. Hoofnagle,Jeffrey R. Whiteaker,Steven A. Carr,Eric Kuhn,Tao Liu,Sam A. Massoni,Stefani N. Thomas,Raymond R. Townsend,Lisa J. Zimmerman,Emily S. Boja,Jing Chen,Daniel L. Crimmins,Sherri R. Davies,Yuqian Gao,Tara Hiltke,Karen A. Ketchum,Christopher R. Kinsinger,Mehdi Mesri,Matthew R. Meyer,Wei-Jun Qian,Regine M. Schoenherr,Mitchell G. Scott,Tujin Shi,Gordon Whiteley,John A. Wrobel,Chaochao Wu,Brad Ackermann,Ruedi Aebersold,David R. Barnidge,David M. Bunk,Nigel J. Clarke,Jordan B. Fishman,Russ P. Grant,Ulrike Kusebauch,Mark M. Kushnir,Mark S. Lowenthal,Robert L. Moritz,Hendrik Neubert,Scott D. Patterson,Alan L. Rockwood,John C. Rogers,Ravinder J. Singh,Jennifer E. Van Eyk,Steven H. Wong,Shucha Zhang,Daniel W. Chan,Xian Chen,Matthew J. Ellis,Daniel C. Liebler,Karin D. Rodland,Henry Rodriguez,Richard D. Smith,Zhen Zhang,Hui Zhang,Amanda G. Paulovich +54 more
TL;DR: The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling.
Journal ArticleDOI
Sequential protein and peptide immunoaffinity capture for mass spectrometry-based quantification of total human β-nerve growth factor
Hendrik Neubert,David Muirhead,Musarat Kabir,Christopher Grace,Adriaan Cleton,Rosalin Arends +5 more
TL;DR: This is the first report of clinical trial implementation of a MS-based assay that uses sequential protein and peptide immunoaffinity capture for protein target quantitation and now provides a bioanalytical platform for the development of similar assays for other protein targets.
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Targeted quantitative proteomics for the analysis of 14 UGT1As and -2Bs in human liver using NanoUPLC-MS/MS with selected reaction monitoring
TL;DR: A multiplexed method using nanoLC-MS/MS and multiple reaction monitoring to quantify 14 UGT1As and UGT2Bs in liver matrices provides novel UGT expression data in human liver that will benefit rational approaches to evaluate metabolism in drug development.
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Highly specific and sensitive measurements of human and monkey interleukin 21 using sequential protein and tryptic peptide immunoaffinity LC-MS/MS.
TL;DR: IL-21 was not detected in serum from normal healthy volunteers or from autoimmune disease patients, however, IL-21 levels were quantified in cynomolgus monkey spleen and colon tissue and normal and inflammatory bowel disease (IBD) human colon tissue as well as hyperplasia human tonsils.