Kalin Mayberry

TL;DR: This article used ABE8e-NRCH to convert the β-globin allele (HBBS) into Makassar βglobin (HBBG), a non-pathogenic variant.

TL;DR: It is shown that loss of the autophagy-activating Unc-51–like kinase 1 (Ulk1) gene in β-thalassemic mice reduces autophagic clearance of α-globin in red blood cell precursors and exacerbates disease phenotypes, whereas inactivation of the canonical autophaky-related 5 (Atg5) gene has relatively minor effects.

TL;DR: It is shown that the E3 ubiquitin ligase FBXO11 relieves PRC2-mediated repression during erythroid maturation by targeting its newly identified substrate BAHD1, an H3K27me3 reader that recruits transcriptional co-repressors.

TL;DR: The findings provide practical information on the genomic structures of two common mouse SCD models, illustrate their limitations for analyzing therapies to induce HbF and confirm the importance of distal DNA elements in human globin regulation.

TL;DR: Sharma et al. as discussed by the authors used ABE-based base editors to install precise nucleotide substitutions without double-stranded break (DSBs) in the tandem duplicated γ-globin genes, which can result in loss or inversion of the intervening genetic material and rearrangements.