Jonathan Yen

TL;DR: It is reported here that butyrate, a naturally occurring fatty acid commonly used as a nutritional supplement and differentiation agent, greatly enhances the efficiency of induced pluripotent stem (iPS) cell derivation from human adult or fetal fibroblasts and provides an efficient method for reprograming various human adult somatic cells, including cells from patients that are more refractory to reprogramming.

TL;DR: It is shown that n -myc can substitute for c-myc and that drug selection is dispensable for reprogramming of fibroblasts to pluripotent stem cells, and that the resulting induced pluripotency cells contribute extensively to teratomas and chimeras.

TL;DR: In primary human T cells, ABE8s achieve 98–99% target modification, which is maintained when multiplexed across three loci, and in human CD34 + cells, Abe8 can recreate a natural allele at the promoter of the γ-globin genes HBG1 and HBG2 with up to 60% efficiency.

TL;DR: This work inhibits the cell-labeling activity of tetraacetyl-N-azidoacetylmannosamine (Ac4ManAz) by converting its anomeric acetyl group to a caged ether bond that can be selectively cleaved by cancer-overexpressed enzymes and thus enables the overexpression of azido groups on the surface of cancer cells.

TL;DR: This article used ABE8e-NRCH to convert the β-globin allele (HBBS) into Makassar βglobin (HBBG), a non-pathogenic variant.