Khoa Le

TL;DR: The availability of this set of antibodies expands the number of vaccine targets on HIV and provides reagents to characterize the native envelope spike, an important property given increasing evidence that cleavage is required for vaccine candidates that seek to mimic the functional HIV envelope spike.

TL;DR: Combined structural studies of PGT 135, PGT 128 and 2G12 show that this Asn332-dependent antigenic region is highly accessible and much more extensive than initially appreciated, which allows for multiple binding modes and varied angles of approach; thereby it represents a supersite of vulnerability for antibody neutralization.

TL;DR: The use of a recombinant HIV envelope trimer as an affinity reagent to isolate quaternary-dependent bnAbs from the peripheral blood mononuclear cells of a chronically infected donor highlights the utility of BG505 SOSIP and reveals a mosaic of antibody responses against the trimer apex within a clonal family.

TL;DR: Functional and genetic characteristics indicate that the boosted mAbs are consistent with partially mature VRC01-class antibodies and place them on a maturation trajectory that leads toward matureVRC 01-class bnAbs, and the results show how reductionist sequential immunization can guide maturation of HIV bnAb responses.

TL;DR: This work found that some bnmAbs can use alternate N-linked glycans in the absence of the N332 glycan site and therefore neutralize a substantial number of viruses lacking the site, and indicated that a diverse response against the high-mannose patch may provide near-equivalent coverage as a combination of bnm Abs targeting multiple epitopes.