Kirstin Rau

TL;DR: The results indicate that high-throughput yeast two-hybrid interactions for human proteins are more precise than literature-curated interactions supported by a single publication, suggesting that HT-Y2H is suitable to map a significant portion of the human interactome.

TL;DR: It is shown that the high-affinity interacting protein ASPL efficiently promotes p97 hexamer disassembly, resulting in the formation of stable p97:ASPL heterotetramers, and that engineered eUBX polypeptides can induce cell death, providing a rationale for developing anti-cancer polyPEptide inhibitors that may target p97 activity.

TL;DR: DULIP is a sensitive and reliable method of great utility for systematic interactome research that can be applied for interaction screening and validation of PPIs in mammalian cells and permits the specific analysis of mutation-dependent binding patterns.

TL;DR: A double‐readout bioluminescence‐based two‐hybrid technology, termed LuTHy, is presented, which provides two quantitative scores in one experimental procedure when testing binary interactions, and is broadly applicable for detecting the effects of small molecules or disease‐causing mutations on PPIs.

TL;DR: A novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection and it is shown that this parallelized screening procedure and the global inspection of Y2h interaction data are uniquely suited to define specific PPI patterns and their alteration by disease-causing mutations in huntingtin and ataxin-1.