Kristofer Davie

TL;DR: A single-cell transcriptome atlas of the entire adult Drosophila melanogaster brain sampled across its lifespan is presented, allowing comprehensive exploration of all transcriptional states of an entire aging brain.

TL;DR: A detailed protocol for pySCENIC: a faster, container-based implementation in Python, resulting in a tenfold increase in speed, and has been packaged into containers for ease of use is described.

TL;DR: As an unsupervised Bayesian framework, cisTopic classifies regions in scATAC-seq data into regulatory topics, which are used for clustering and provides insight into the mechanisms underlying regulatory heterogeneity in cell populations.

TL;DR: A single-cell atlas of the adult fly, Tabula Drosophilae, that includes 580,000 nuclei from 15 individually dissected sexed tissues as well as the entire head and body, annotated to >250 distinct cell types is presented, providing an in-depth analysis of cell type–related gene signatures and transcription factor markers, as as sexual dimorphism, across the whole animal.

TL;DR: It is shown that FAIRE-seq and ATAC-seq based open chromatin profiling, combined with motif discovery, is a straightforward approach to identify functional genomic regulatory regions, master regulators, and gene regulatory networks controlling complex in vivo processes.