Lars Dölken

TL;DR: An improved approach to separate total cellular RNA into newly transcribed and preexisting RNA following 10-15 min of metabolic labeling is developed and a previously undisclosed highly connected network of short-lived transcripts selectively down-regulated by IFNgamma is identified.

TL;DR: DTA realistically monitors the dynamics in mRNA metabolism that underlie gene regulatory systems, and can monitor the cellular response to osmotic stress with higher sensitivity and temporal resolution than standard transcriptomics.

TL;DR: This work shows that different regulatory patterns between functionally similar proteins are characterized by differences in the half-life of the corresponding transcripts and can be identified by measuring RNA half- life, and predicts more than 100 protein families which show such differential regulatory patterns in both species.

TL;DR: It is found that HSV-1 triggers the disruption of transcription termination of cellular, but not viral, genes, resulting in extensive transcription for tens of thousands of nucleotides beyond poly(A) sites and into downstream genes, leading to novel intergenic splicing between exons of neighbouring cellular genes.

TL;DR: It is shown that the cyclin-dependent kinase 8 (CDK8) module of the Mediator complex phosphorylated regulatory sites within the TADs of STAT1, STAT3, and STAT5, including S727 within the STAT1 TAD in the interferon (IFN) signaling pathway, suggesting a general role for CDK8 in STAT-mediated transcription.