Lukas Lomb

TL;DR: Interpretable diffraction data is recorded from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

TL;DR: A complete set of structure factors has been extracted from hundreds of thousands of femtosecond single-shot X-ray microdiffraction patterns taken from randomly oriented nanocrystals, and the method of Monte Carlo integration over crystallite size and orientation was applied.

TL;DR: This first experimental demonstration of cryptotomography extended the expansion-maximization-compression framework to accommodate unmeasured fluctuations in photon fluence and loss of data due to saturation or background scatter to realize single-shot diffraction imaging of single biomolecules.

TL;DR: This work presents an unsupervised method able to sort experimental snapshots without recourse to templates, specific noise models, or user-directed learning and shows 90% agreement with manual classification.

TL;DR: In this article, the authors modify iterative reconstruction methods to improve tolerance to noise in single-shot coherent diffractive imaging at X-ray free-electron laser facilities, which is limited by the low signal-to-noise level of diffraction data at high scattering angles.