M
M. Leslie Fulcher
Researcher at University of North Carolina at Chapel Hill
Publications - 12
Citations - 2463
M. Leslie Fulcher is an academic researcher from University of North Carolina at Chapel Hill. The author has contributed to research in topics: Medicine & Mucin. The author has an hindex of 8, co-authored 10 publications receiving 1766 citations.
Papers
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Journal ArticleDOI
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract.
Yixuan J. Hou,Kenichi Okuda,Caitlin E. Edwards,David R. Martinez,Takanori Asakura,Kenneth H. Dinnon,Takafumi Kato,Rhianna E. Lee,Boyd Yount,Teresa M. Mascenik,Gang Chen,Kenneth N. Olivier,Andrew J. Ghio,Longping V. Tse,Sarah R. Leist,Lisa E. Gralinski,Alexandra Schäfer,Hong Dang,Rodney C. Gilmore,Satoko Nakano,Ling Sun,M. Leslie Fulcher,Alessandra Livraghi-Butrico,Nathan I. Nicely,Mark J. Cameron,Cheryl M. Cameron,David J. Kelvin,David J. Kelvin,Aravinda M. de Silva,David M. Margolis,Alena J. Markmann,Luther A. Bartelt,Ross E. Zumwalt,Fernando J. Martinez,Steven P. Salvatore,Alain C. Borczuk,Purushothama Rao Tata,Vishwaraj Sontake,Adam J. Kimple,Ilona Jaspers,Wanda K. O'Neal,Scott H. Randell,Richard C. Boucher,Ralph S. Baric +43 more
TL;DR: The nasal susceptibility to SARS-CoV-2 with likely subsequent aspiration-mediated virus seeding to the lung in SARS/COVID-19 pathogenesis is highlighted and reagents provide a foundation for investigations into virus-host interactions in protective immunity, host susceptibility, and virus pathogenesis.
Book ChapterDOI
Well-differentiated human airway epithelial cell cultures.
Journal ArticleDOI
Potentiator ivacaftor abrogates pharmacological correction of ΔF508 CFTR in cystic fibrosis
Deborah M. Cholon,Nancy L. Quinney,M. Leslie Fulcher,Charles R. Esther,Jhuma Das,Nikolay V. Dokholyan,Scott H. Randell,Richard C. Boucher,Martina Gentzsch +8 more
TL;DR: It is found that both acute and chronic treatment with VX-770 improved CFTR function in cells with the G551D mutation, consistent with clinical studies, and these findings demonstrate that chronic treatmentWith CFTR potentiators and correctors may have unexpected effects that cannot be predicted from short-term studies.
Book ChapterDOI
Human nasal and tracheo-bronchial respiratory epithelial cell culture
TL;DR: This chapter gives detailed protocols for tissue procurement, cell isolation, production of complex media, and cell culture initiation and maintenance needed for hAE cell ALI cultures with non-proprietary reagents.
Book ChapterDOI
Primary epithelial cell models for cystic fibrosis research.
TL;DR: This chapter gives protocols enabling creation of well-differentiated primary CF and non-CF airway epithelial cell cultures with non-proprietary reagents and discusses the production of retroviral and lentiviral vectors.