M
Makoto R. Hara
Researcher at Duke University
Publications - 21
Citations - 4870
Makoto R. Hara is an academic researcher from Duke University. The author has contributed to research in topics: Signal transduction & Beta-Arrestins. The author has an hindex of 18, co-authored 20 publications receiving 4484 citations. Previous affiliations of Makoto R. Hara include Johns Hopkins University School of Medicine & Johns Hopkins University.
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Journal ArticleDOI
S-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 binding
Makoto R. Hara,Nishant Agrawal,Sangwon F. Kim,Matthew B. Cascio,Masahiro Fujimuro,Yuji Ozeki,Masaaki Takahashi,Jaime H. Cheah,Stephanie Tankou,Lynda D. Hester,Christopher D. Ferris,S. Diane Hayward,Solomon H. Snyder,Akira Sawa +13 more
TL;DR: A signalling pathway in which nitric oxide generation that follows apoptotic stimulation elicits S-nitrosylation of GAPDH, which triggers binding to Siah1 (an E3 ubiquitin ligase), nuclear translocation and apoptosis, which is prevented by NO deletion is reported.
Journal ArticleDOI
Distinct phosphorylation sites on the β(2)-adrenergic receptor establish a barcode that encodes differential functions of β-arrestin.
Kelly N. Nobles,Kunhong Xiao,Seungkirl Ahn,Arun K. Shukla,Arun K. Shukla,Christopher M. Lam,Sudarshan Rajagopal,Ryan T. Strachan,Teng Yi Huang,Erin Ashley Bressler,Makoto R. Hara,Sudha K. Shenoy,Steven P. Gygi,Robert J. Lefkowitz,Robert J. Lefkowitz +14 more
TL;DR: It is proposed that the distinct phosphorylation patterns established by different GRKs establish a “barcode” that imparts distinct conformations to the recruited β-arrestin, thus regulating its functional activities.
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Nitric Oxide Regulates Exocytosis by S-Nitrosylation of N-ethylmaleimide-Sensitive Factor
Kenji Matsushita,Craig N. Morrell,Béatrice Cambien,Shui Xiang Yang,Munekazu Yamakuchi,Clare Bao,Makoto R. Hara,Richard A. Quick,Wangsen Cao,Brian O'Rourke,John M. Lowenstein,Jonathan Pevsner,Denisa D. Wagner,Charles J. Lowenstein +13 more
TL;DR: It is shown that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and thrombosis, by regulating the activity of N-ethylmaleimide-sensitive factor (NSF).
Journal ArticleDOI
Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis
Nilkantha Sen,Makoto R. Hara,Makoto R. Hara,Michael D. Kornberg,Matthew B. Cascio,Byoung-Il Bae,Neelam Shahani,Bobby Thomas,Ted M. Dawson,Valina L. Dawson,Solomon H. Snyder,Akira Sawa +11 more
TL;DR: It is shown that nuclear GAPDH is acetylated at Lys 160 by the acetyltransferase p300/CREB binding protein (CBP) through direct protein interaction, which in turn stimulates the acetolation and catalytic activity of p 300/CBP.
Journal ArticleDOI
GAPDH mediates nitrosylation of nuclear proteins
Michael D. Kornberg,Nilkantha Sen,Makoto R. Hara,Makoto R. Hara,Krishna R. Juluri,Judy V. Nguyen,Adele M. Snowman,Lindsey Law,Lynda D. Hester,Solomon H. Snyder +9 more
TL;DR: It is shown that SNO–GAPDH physiologically transnitrosylates nuclear proteins, including the deacetylating enzyme sirtuin-1 (SIRT1), histone de acetylase-2 (HDAC2) and DNA-activated protein kinase (DNA-PK), which suggest that protein–protein transfer of nitric oxide groups may be a general mechanism in cellular signal transduction.