Maren Kopp

TL;DR: The complete genome sequence of the model Sorangium strain S. cellulosum So ce56 is reported, which produces several natural products and has morphological and physiological properties typical of the genus, and the circular genome is the largest bacterial genome sequenced to date.

TL;DR: The first electroporation protocol for a strain of the genus Sorangium is presented, and the transposon library was screened for disorazol‐negative mutants, leading to the identification of the corresponding trans‐acyltransferase core biosynthetic gene cluster.

TL;DR: Using biparental and triparental mating, methodologies for DNA transfer from Escherichia coli via conjugation for the genome sequencing model strain So ce56 and the secondary metabolite multiproducing strain so ce12 are developed.

TL;DR: The finding that sorangicin A exhibits significantly greater tolerance to mutation-induced changes in the shape of the RNAP binding pocket relative to rifampicin, which is likely due its greater conformational flexibility, suggests that structural analogues of the Sorangicins could have utility in tackling strains of tuberculosis that are rifampsicin resistant.

TL;DR: This study supports the analysis by the reconstitution in vitro of the biosynthesis of the pyrrole carboxylic starter unit along with gene inactivation and reports the generation of four novel deshydroxy leupyrrin analogues by genetic engineering of the pathway.