Markus Beier

TL;DR: A chemistry was developed that permits on DNA-arrays both the covalent immobilisation of pre-fabricated nucleic acids-such as oligonucleotides, PCR-products or peptide nucleic acid oligomers-and the in situ synthesis of such compounds on either glass or polypropylene surfaces.

TL;DR: In a multicenter study, the expression profiles of 863 microRNAs were determined by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and this 'miRNome' was validated by quantitative real-time PCR.

TL;DR: This procedure prevents the overall background signal that occurs with the frequently applied aqueous solvent 1-methyl-2-pyrrolidone in borate buffer because of DNA that re-dissolves from spots during the blocking process, only to bind again across the entire glass surface.

TL;DR: This work shows that the genomic region of interest can be enriched by a mean factor of 2169 compared with the coverage of the whole genome, resulting in high sequence coverage of selected disease genes and allowing us to define the genetic pathogenesis of cardiomyopathies in a single sequencing run.

TL;DR: The results imply that the thermodynamic properties of oligonucleotide hybridization are by far not yet understood, and recommend against the application of probe design software tools that use thermodynamic parameters to assess probe quality for species identification.