Sae Okazaki

TL;DR: A rationally engineered SpCas9 variant (SpCas9-NG) that can recognize relaxed NG PAMs is reported, which is a powerful addition to the CRISPR-Cas9 genome engineering toolbox and will be useful in a broad range of applications, from basic research to clinical therapeutics.

TL;DR: In this paper, the authors developed a platform that allows "CRISPR-based amplification-free digital RNA detection (SATORI)" by combining CRISPRCas13-based RNA detection and microchamber-array technologies.

TL;DR: Cryo-electron microscopy structure of Cas12f1 (also known as Cas14a) in complex with a guide RNA and its target DNA revealed that two Cas 12f1 molecules assemble with the single guide RNA to recognize the double-stranded DNA target, thereby explaining how the miniature Cas12 f1 enzyme achieves RNA-guided DNA cleavage as an "asymmetric homodimer."

TL;DR: The 2.4-Å-resolution structure of HeR from an uncultured Thermoplasmatales archaeon SG8-52-1 shows that it adopts a similar fold to that of type I rhodopsin—despite the low sequence identity—but there are also several marked differences that provide insights into heliorhodopin function.

TL;DR: The type III-E CRISPR-Cas effector Cas7-11, with dual RNase activities for pre-crRNA processing and CRRNA-guided target RNA cleavage, is a new platform for bacterial and mammalian RNA targeting as discussed by the authors .