Michael D. Waterfield

TL;DR: The solution structure of the uncomplexed C-terminal SH2 (C-SH2) domain of the p85 alpha subunit of PI 3-kinase has been determined by means of multinuclear, double and triple-resonance NMR experiments and restrained molecular-dynamics simulatedannealing calculations.

TL;DR: The ChiClust tool was used here to order the breast cell lines into groups according to biological characteristics including morphology in vitro and tumour forming ability in vivo, and ChiMap was then used to highlight eight major protein feature‐changes detected between breast cancer cell lines that either do or do not proliferate in nude mice.

TL;DR: The results raise the possibility that a wortmannin-sensitive pathway independent of heteromeric PI 3-kinase is involved in the activation of p70 S6 kinase by insulin.

TL;DR: A two-dimensional differential proteome analysis of replicative senescence in serially passaged rat embryo fibroblasts revealed 49 spots whose expression was altered more than 2-fold, including gelsolin, a candidate tumor suppressor for breast cancer, and α-glucosidase II, a member of the family of glucosidases that includes klotho; a defect in klOTHo expression in mice results in a syndrome that resembles human aging.

TL;DR: The data demonstrate that EGFR and Src together play a central role in oxidative stress-induced phosphorylation, which in turn results in loss of adhesion, morphological changes, and cell damage in epithelial cells, which provides a general model for redox signaling in other cell systems.