N
Nikolai P. Skiba
Researcher at Duke University
Publications - 65
Citations - 4191
Nikolai P. Skiba is an academic researcher from Duke University. The author has contributed to research in topics: Transducin & G protein. The author has an hindex of 25, co-authored 55 publications receiving 3887 citations. Previous affiliations of Nikolai P. Skiba include University of Illinois at Chicago & University of Wisconsin-Madison.
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Journal ArticleDOI
The 2.0 Å crystal structure of a heterotrimeric G protein
TL;DR: The structure of a heterotrimeric G protein reveals the mechanism of the nucleotide-dependent engagement of the α and βγ subunits that regulates their interaction with receptor and effector molecules.
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Molecular Basis for Interactions of G Protein βγ Subunits with Effectors
Carolyn E. Ford,Nikolai P. Skiba,Hyunsu Bae,Yehia Daaka,Eitan Reuveny,Lee R. Shekter,Ramon Rosal,Gezhi Weng,Chii-Shen Yang,Ravi Iyengar,Richard J. Miller,Lily Yeh Jan,Robert J. Lefkowitz,Heidi E. Hamm +13 more
TL;DR: Analysis of the ability of mutants to regulate the activity of calcium and potassium channels, adenylyl cyclase 2, phospholipase C-β2, and β-adrenergic receptor kinase revealed the Gβ residues required for activation of each effector and provides evidence for partially overlapping domains on Gβ for regulation of these effectors.
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Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome
Julia A. Chekanova,Julia A. Chekanova,Brian D. Gregory,Sergei V. Reverdatto,Huaming Chen,Ravi Kumar,Tanya Hooker,Junshi Yazaki,Pinghua Li,Nikolai P. Skiba,Qian Peng,Qian Peng,Jose M. Alonso,Vladimir Brukhin,Ueli Grossniklaus,Joseph R. Ecker,Dmitry A. Belostotsky,Dmitry A. Belostotsky +17 more
TL;DR: It is demonstrated that as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity and a genome-wide atlas of Arabidopsis exosomes targets is presented, which will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes.
Journal ArticleDOI
Molecular Determinants of Selectivity in 5-Hydroxytryptamine1B Receptor-G Protein Interactions
Hyunsu Bae,Kristin A. Anderson,Lori A. Flood,Nikolai P. Skiba,Heidi E. Hamm,Stephen G. Graber +5 more
TL;DR: The studies suggest that the α4 helix and α4-β6 loop region of Gαs are an important region for specific recognition between receptors and Gi family members.
Journal ArticleDOI
Mapping of Effector Binding Sites of Transducin α-Subunit Using Gαt/Gαil Chimeras
TL;DR: Using defined Gα/Gα chimeras, this study individuated the regions on Gα most important for interaction with PDE in the basal and activated states and defined contact sites on G α with the effector enzyme cGMP phosphodiesterase.