Norbert Topf

TL;DR: Conditions to direct hES cells into neurons of midbrain dopaminergic identity are described and high-yield DA neuron derivation was confirmed from three independent hES and two monkey embryonic stem cell lines.

TL;DR: A set of coculture conditions is provided that allows rapid and efficient derivation of most central nervous system phenotypes and transplantation of ES and ntES cell–derived dopaminergic neurons corrected the phenotype of a mouse model of Parkinson disease, demonstrating an in vivo application of therapeutic cloning in neural disease.

TL;DR: The double mutant α1(S270H,L277A)β2γ2S GABAAR was expressed in Xenopus laevis oocytes and human embryonic kidney (HEK)293 cells, and it had near-normal GABA sensitivity, but rapid application of a brief GABA pulse to receptors expressed in HEK293 cells revealed that the deactivation was faster in double mutant than in wild-type receptors.

TL;DR: The data suggest that propofol modulation of HCN channel gating is an important molecular mechanism that can contribute to the depression of central nervous system function and also lead to bradyarrhythmias in patients receiving prop ofol during surgical anesthesia.

TL;DR: It is feasible to genetically modify AM with Ad vectors and to use this strategy to modify the behavior of AM in vivo, and this strategy may be useful in enhancing pulmonary defenses in immunodeficiency states.