Paul Reginato

TL;DR: In this paper, the authors applied untargeted expansion sequencing (ExSeq) to the mouse brain, which yielded the readout of thousands of genes, including splice variants, revealing patterns across multiple cell types, layer-specific cell types across the mouse visual cortex, and the organization and position dependent states of tumor and immune cells in a human metastatic breast cancer biopsy.

TL;DR: In situ genome sequencing (IGS) as discussed by the authors is a method for simultaneously sequencing and imaging genomes within intact biological samples, which can directly connect sequence and structure across length scales from single base pairs to whole organisms.

TL;DR: Untargeted expansion sequencing (ExSeq) is applied to mouse brain, yielding readout of thousands of genes, including splice variants and novel transcripts, and enables highly multiplexed mapping of RNAs, from nanoscale to system scale.

TL;DR: OligoFISSEQ combines Oligopaints with fluorescence in situ sequencing to enable the 3D mapping of many regions across the genome in human cells to interrogate genome organization at improved genomic resolution, and is compatible with immunochemistry and OligoSTORM for super-resolution imaging.

TL;DR: Expansion microscopy of the larval and embryonic zebrafish may enable systematic studies of how molecular components are configured in multiple contexts of interest to neuroscience and developmental biology.