S
Styliani Markoulaki
Researcher at Massachusetts Institute of Technology
Publications - 44
Citations - 8878
Styliani Markoulaki is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Induced pluripotent stem cell & Embryonic stem cell. The author has an hindex of 29, co-authored 43 publications receiving 8207 citations. Previous affiliations of Styliani Markoulaki include Beth Israel Deaconess Medical Center & Tufts University.
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Journal ArticleDOI
Treatment of Sickle Cell Anemia Mouse Model with iPS Cells Generated from Autologous Skin
Jacob H. Hanna,Marius Wernig,Marius Wernig,Styliani Markoulaki,Styliani Markoulaki,Chiao-Wang Sun,Chiao-Wang Sun,Alexander Meissner,Alexander Meissner,John P. Cassady,John P. Cassady,Caroline Beard,Caroline Beard,Tobias Brambrink,Tobias Brambrink,Li-Chen Wu,Li-Chen Wu,Tim M. Townes,Tim M. Townes,Rudolf Jaenisch,Rudolf Jaenisch +20 more
TL;DR: It is shown that mice can be rescued after transplantation with hematopoietic progenitors obtained in vitro from autologous iPS cells, providing proof of principle for using transcription factor–induced reprogramming combined with gene and cell therapy for disease treatment in mice.
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Direct Reprogramming of Terminally Differentiated Mature B Lymphocytes to Pluripotency
Jacob H. Hanna,Styliani Markoulaki,Patrick Schorderet,Bryce W. Carey,Caroline Beard,Marius Wernig,Menno P. Creyghton,Eveline J. Steine,John P. Cassady,Ruth K. Foreman,Christopher J. Lengner,Jessica Dausman,Rudolf Jaenisch +12 more
TL;DR: This study provides definite proof for the direct nuclear reprogramming of terminally differentiated adult cells to pluripotency through transgenic and inducible expression of transcription factors.
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Single-Cell Expression Analyses during Cellular Reprogramming Reveal an Early Stochastic and a Late Hierarchic Phase
Yosef Buganim,Dina A. Faddah,Albert W. Cheng,Elena Itskovich,Styliani Markoulaki,Kibibi Ganz,Sandy Klemm,Alexander van Oudenaarden,Alexander van Oudenaarden,Rudolf Jaenisch +9 more
TL;DR: In this paper, the authors utilized two gene expression technologies to profile 48 genes in single cells at various stages during the reprogramming process and found that expression of Esrrb, Utf1, Lin28, and Dppa2 is a better predictor for cells to progress into iPSCs than expression of the previously suggested reprogrammer markers Fbxo15, Fgf4, and Oct4.
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Reprogramming of murine and human somatic cells using a single polycistronic vector
Bryce W. Carey,Styliani Markoulaki,Jacob H. Hanna,Kris Saha,Qing Gao,Maisam Mitalipova,Rudolf Jaenisch +6 more
TL;DR: A novel approach to reduce the number of viruses necessary to reprogram somatic cells by delivering reprogramming factors in a single virus using 2A “self-cleaving” peptides, which support efficient polycistronic expression from a single promoter.
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A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types
Marius Wernig,Christopher J. Lengner,Jacob H. Hanna,Michael A. Lodato,Eveline J. Steine,Ruth K. Foreman,Judith Staerk,Styliani Markoulaki,Rudolf Jaenisch +8 more
TL;DR: It is demonstrated that various induction levels of the reprogramming factors can induce pluripotency, the duration of transgene activity directly correlates with reprograming efficiency, and cells from many somatic tissues can be reprogrammed and different cell types require different induction levels.