P
Phill Rogers
Researcher at European Bioinformatics Institute
Publications - 6
Citations - 1554
Phill Rogers is an academic researcher from European Bioinformatics Institute. The author has contributed to research in topics: Reverse transfection & Gene. The author has an hindex of 5, co-authored 6 publications receiving 1475 citations.
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Journal ArticleDOI
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes
Beate Neumann,Thomas Walter,Jean-Karim Hériché,Jutta Bulkescher,Holger Erfle,Christian Conrad,Phill Rogers,Ina Poser,Michael Held,Urban Liebel,Cihan Cetin,Frank Sieckmann,Gregoire Pau,Rolf Kabbe,Annelie Wünsche,Venkata P. Satagopam,Michael H.A. Schmitz,Catherine Chapuis,Daniel W. Gerlich,Reinhard Schneider,Roland Eils,Wolfgang Huber,Jan-Michael Peters,Anthony A. Hyman,Richard Durbin,Rainer Pepperkok,Jan Ellenberg +26 more
TL;DR: This study carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival.
Journal ArticleDOI
High-throughput RNAi screening by time-lapse imaging of live human cells.
Beate Neumann,Michael Held,Urban Liebel,Holger Erfle,Phill Rogers,Rainer Pepperkok,Jan Ellenberg +6 more
TL;DR: This work has developed an automated platform for high-content RNAi screening by time-lapse fluorescence microscopy of live HeLa cells expressing histone-GFP to report on chromosome segregation and structure and validated this method in a pilot screen assaying cell division.
Journal ArticleDOI
Reverse transfection on cell arrays for high content screening microscopy.
Holger Erfle,Beate Neumann,Urban Liebel,Phill Rogers,Michael Held,Thomas Walter,Jan Ellenberg,Rainer Pepperkok +7 more
TL;DR: A robust protocol for the reverse transfection of cells on small interfering (siRNA) arrays, which, in combination with multi-channel immunofluorescence or time-lapse microscopy, is suitable for genome-wide RNA interference (RNAi) screens in intact human cells.
Journal ArticleDOI
Work flow for multiplexing siRNA assays by solid-phase reverse transfection in multiwell plates.
TL;DR: It is concluded that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays.
Journal ArticleDOI
EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase
Justus Tegha-Dunghu,Beate Neumann,Simone Reber,Roland Krause,Holger Erfle,Thomas Walter,Michael Held,Phill Rogers,Kerstin Hupfeld,Thomas Ruppert,Jan Ellenberg,Oliver J. Gruss +11 more
TL;DR: The proteomic identification screen combined with sensitive phenotypic analysis provides a reliable platform for the identification and characterisation of proteins important for correct cell division.