Jutta Bulkescher

TL;DR: This study carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival.

TL;DR: The approaches described here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian cells and led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting complex and the γ-tubulin ring complex—large complexes that are essential for spindle assembly and chromosome segregation.

TL;DR: High-throughput (HT)-FCS is presented, which automates screening and time-lapse acquisition of FCS data at specific subcellular locations and subsequent data analysis and expects that throughput and robustness will make HT-FCS a broadly applicable technology for characterizing protein network dynamics in cells.

TL;DR: The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells, allowing us to statistically analyze biological processes in detail.

TL;DR: It is concluded that solid-phase reverse transfection in multiwell plates is a cost-efficient and flexible tool for multiplexing cellular assays.