Rafael Radi

TL;DR: Spectroscopic characterization of two nitrated variants of horse heart cytochrome c by using optical spectroscopy studies and NMR provides new insights into how a remote post-translational modification in cy tochrome c such as tyrosine nitration triggers profound structural changes in the heme ligation and microenvironment and impacts in protein function.

TL;DR: When bovine aortic endothelial cells (BAEC) were incubated with HBSS and PHPA alone, 50% less fluorescence was observed than when HRP was added, which correlated well with a weak range of buffering capacity of (PHPA)2.

TL;DR: The results help to rationalize MnSOD tyrosine nitration observed in inflammatory conditions in vivo in the presence of low molecular weight scavengers such as glutathione that otherwise would completely consume nitrogen dioxide and prevent nitration reactions.

TL;DR: It is hypothesize that in biological systems the presence of superoxide dismutase (SOD) and the facile transmembrane diffusion of (*)NO preclude accumulation of O(2)*(-) and(*)NO radicals under flux ratios different from one, preventing the secondary reactions that result in the inhibition of 3-nitrotyrosine formation.

TL;DR: The endogenous presence of NO2-FA in both EVOO and fresh olives was demonstrated by mass spectrometry and affirm a role for these species as signaling mediators in mammalian and plant lipids.