Rafael Radi

TL;DR: It is proposed that desferrioxamine can inhibit the oxidative chemistry of peroxynitrite by reaction of the hydroxamic acid moieties with trans-peroxyn itrous acid.

TL;DR: Analysis of kinetic and mechanistic aspects that allow rationalizing the biological fate of peroxynitrite from data obtained by a combination of methods that include fast kinetic techniques, electron paramagnetic resonance and kinetic simulations provides a handle to predict the preferential fate and steady-state levels in living systems.

TL;DR: These kinetic characteristics qualify the peroxiredoxins as moderately efficient devices to detoxify hydroperoxides, which is pivotal to organisms devoid of more efficient peroxidases, and as most relevant to the detoxification of peroxynitrite.

TL;DR: It is shown that neither commercial nor pure MnTBAP could substitute for SOD enzyme in a SOD-deficient Escherichia coli model, whereas MnTE-2-PyP-treated SOD -deficient E. coli grew as well as a wild-type strain, which indicates that Mn TBAP does not act as an SOD mimic in vivo.

TL;DR: Kinetics of the reaction of peroxynitrite with the different thiol-containing components of the cytosolic tryparedoxin peroxidase system in T. brucei and T. cruzi are studied, supporting that these peroxiredoxins would be not only a preferential target for peroxnitrite reactivity but also be able to act catalytically in peroxlynitrite decomposition in vivo.