Richard James

TL;DR: Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases, and 54% of the predicted genes had significant similarity to known genes, and other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, and the frequent occurrence of clustered gene families.

TL;DR: The results show that the population of an intermediate in the refolding of the immunity protein structure is defined by the precise amino acid sequence rather than the global stability of the protein.

TL;DR: The BRP is involved in release of the colicin-immunity protein complex from the producing cell and causes partial cell lysis upon strong induction of the SOS promoter.

TL;DR: The crystal structure of the cytotoxic endonuclease domain from the bacterial toxin colicin E9 in complex with its cognate immunity protein Im9 reveals that the inhibitor does not bind at the active site, but rather at an adjacent position leaving theActive site exposed yet unable to bind DNA because of steric and electrostatic clashes with incoming substrate.

TL;DR: The 1.7 A crystal structure of the 24.5 kDa complex formed between the endonuclease domain of colicin E9 and its cognate immunity protein Im9 is reported, which provides a molecular rationale for this mechanism and highlights how specificity is achieved by very different interactions in the two complexes.