Robert Hänsel-Hertsch

TL;DR: Advances in the detection and mapping of G4 structures in the human genome and in biologically relevant contexts provide important new insights into the functions of G 4 structures in, for example, the regulation of transcription and genome stability, and uncover their potential relevance for cancer therapy.

TL;DR: The development of G4 ChIP–seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach, shows that regulatory, nucleosome-depleted chromatin and elevated transcription shape the endogenous human G4 DNA landscape.

TL;DR: This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing.

TL;DR: It is shown that i‐motifs formed from naturally occurring C‐rich sequences in the human genome are stable and persist in the nuclei of living human cells, the first to interlink the stability of DNA i‐Motifs in vitro with their stability in’vivo.

TL;DR: Evidence is provided that G-quadruplex (G4) DNA secondary structures are genomic features that influence methylation at CGIs and it is proposed that G4 formation sequesters DNMT1 thereby protecting certain CGIs from methylation and inhibiting local methylation.