Giovanni Marsico

TL;DR: It is estimated that escape of siRNAs from endosomes into the cytosol occurs at low efficiency (1–2%) and only during a limited window of time when the LNPs reside in a specific compartment sharing early and late endosomal characteristics.

TL;DR: The development of G4 ChIP–seq, an antibody-based G4 chromatin immunoprecipitation and high-throughput sequencing approach, shows that regulatory, nucleosome-depleted chromatin and elevated transcription shape the endogenous human G4 DNA landscape.

TL;DR: A mathematical model of endosome dependency on Rab5 was developed and validated by titrating down all three Rab5 isoforms in adult mouse liver using state-of-the-art RNA interference technology and found the endocytic system was resilient to depletion of Rab5 and collapsed only when Rab5 decreased to a critical level.

TL;DR: It is shown for the first time that the resection of the autonomic nerves stops the progression of PD pathology and that this progression is based on the transneuronal and retrograde axonal transport of alpha-synuclein.

TL;DR: RedBS-Seq is introduced, a quantitative method to decode 5fC in DNA at single-base resolution, based on a selective chemical reduction of 5 fC to 5hmC followed by bisulfite treatment, to generate the first combined genomic map of 5-methylcytosine,5hmC and 5fF in mouse embryonic stem cells.