Roland Benz

TL;DR: The binding component (Vip1Ac) of the ADP-ribosylating vegetative insecticidal protein of Bacillus thuringiensis HD201 was isolated from the supernatant of cell cultures and Mass spectroscopic analysis of this high molecular mass band identified it as Vip1 Ac.

TL;DR: The cell wall of the gram-positive Corynebacterium glutamicum was prepared and an ion-permeable channel with a single-channel conductance of about 6 nS in 1 M KCl was prepared, indicating that it is a water-filled channel wide enough to allow unhindered diffusion of ions.

TL;DR: The adsorption of [W(CO)(5)CN](-) was significantly reduced by phloretin, which is in reasonable agreement with the known phlrotin-induced effects on artificial and biological membranes, and is consistent with the assumption that the binding of phLoretin reduces the intrinsic dipole potential of the plasma membrane.

TL;DR: Southern blots demonstrated that only the chromosomes of corynebacteria contain homologous sequences to porA; no hybridization could be detected with DNA from other mycolata.

TL;DR: The organization of P66 as a possible octamer was confirmed by Blue Native PAGE and immunoblot analysis, which both demonstrated that P66 forms a complex with a mass of approximately 460 kDa, and two dimension SDS PAGE revealed that P 66 is the only polypeptide in the complex.