Showing papers in "Developmental Cell in 2015"

TL;DR: It is suggested that mutant phenotypes become the standard metric to define gene function in zebrafish, after which Morpholinos that recapitulate respective phenotypes could be reliably applied for ancillary analyses.

TL;DR: FAs engage in complex trafficking itineraries regulated by cytoplasmic lipases, autophagy, and mitochondrial fusion dynamics, ensuring maximum oxidative metabolism and avoidance of FA toxicity in starved cells.

TL;DR: It is reported that OST1 (open stomata 1), a well-known Ser/Thr protein kinase in ABA signaling, acts upstream of CBFs to positively regulate freezing tolerance, thus suppressing HOS1-mediated ICE1 degradation under cold stress.

TL;DR: These findings support a conserved transcription factor foundation for naive pluripotency while revealing species-specific regulatory features of lineage segregation in rodents.

TL;DR: A simple and scalable CRISPR-based vector system for tissue-specific gene inactivation in zebrafish and constitutes a unique tool to spatially control gene knockout and greatly broadens the scope of loss-of-function studies inZebrafish.

TL;DR: This work demonstrates that proximity labeling can be applied to proteomics of non-membrane-enclosed organelles and suggests that proteomics profiling of cilia will enable a rapid and powerful characterization of ciliopathies.

TL;DR: The identification of a multisubunit complex named BORC that regulates lysosome positioning is reported, which comprises eight subunits that functions to recruit the small GTPase Arl8 and promotes the kinesin-dependent movement of lysOSomes toward the plus ends of microtubules in the peripheral cytoplasm.

TL;DR: It is shown that mechanical force exerted by signal-sending cells is required for ligand-induced Notch activation and established that force-induced proteolysis can act as a mechanism of cellular mechanotransduction.

TL;DR: This work shows that in the Arabidopsis root meristem, the WOX5 protein moves from the root niche organizer, the quiescent center, into the columella stem cells, where it directly represses the transcription factor gene CDF4.

TL;DR: It is shown that myelin basic protein, a protein essential for CNS myelin wrapping whose role has been unclear, is required for actin disassembly, and its loss phenocopies loss of actindisassembly proteins are required for normal wrapping.

TL;DR: Primary cilia interpret vertebrate Hedgehog (Hh) signals and regulate ciliary membrane phosphoinositide composition, and Tulp3 reads out ciliaryosphoinositides to control ciliary protein localization, enabling Hh signaling.

TL;DR: It is shown that chloroplasts send out dynamic tubular extensions called stromules during innate immunity or exogenous application of the pro-defense signals, hydrogen peroxide (H2O2) and salicylic acid and these connections correlate with an accumulation of chloroplast-localized NRIP1 defense protein and H 2O2 in the nucleus.

TL;DR: The molecular mechanisms of myelination are discussed, along with new perspectives on oligodendrocyte plasticity and myelin remodeling of the developing and adult CNS.

TL;DR: It is shown that the switch from E- to N-cadherin during EMT is essential for acquisition of CIL behavior, which provides insight into the balance of physical forces that contributes to CIL in cells in vivo.

TL;DR: Antagonism of the Arp2/3 complex by profilin-1 in cells appears to maintain actin homeostasis by balancing Arp1/2 complex-dependent and -independent actin assembly pathways.

TL;DR: This work shows that the LPR1-PDR2 module facilitates, upon Pi limitation, cell-specific apoplastic Fe and callose deposition in the meristem and elongation zone of primary roots, and links callose-regulated cell-to-cell signaling in root meristems to the perception of an abiotic cue.

TL;DR: The Myod-LncMyoD-IMP2 pathway elucidates a mechanism as to how MyoD blocks proliferation to create a permissive state for differentiation.

TL;DR: This review summarizes the state-of-the-art of vessel pruning and regression with a focus on the cellular processes and the molecular regulators of vessel maintenance and regression.

TL;DR: The link between ciliary regulation of phosphoinositides by INPP5E and Shh regulation via ciliary trafficking of TULP3/Gpr161 is revealed and mechanistic insight into ciliary alterations found in Joubert and MORM syndromes resulting from IN PP5E mutations is provided.

TL;DR: Using a combination of cell biological approaches and in vitro reconstitution of competition between actin assembly factors, it is found that the small G-actin binding protein profilin directly inhibits Arp2/3 complex-mediated actinAssembly.

TL;DR: It is shown using transgenic zebrafish that inhibition of cardiac innervation leads to reduction of myocyte proliferation following injury, and pharmacological inhibition of cholinergic nerve function reduces cardiomyocyte proliferation in the injured hearts of both zebra fish and neonatal mice.

TL;DR: DNA sequence-dependent binding of CENP-B within α-satellite repeats is required to stabilize optimal centromeric levels of C ENP-C, and data demonstrate a DNA sequence-specific enhancement by CENp-B of the fidelity of epigenetically defined human centromere function.

TL;DR: A model for organ wasting in adult Drosophila is developed, whereby overproliferation induced by activation of Yorkie, the Yap1 oncogene ortholog, in intestinal stem cells leads to wasting of the ovary, fat body, and muscle.

TL;DR: It is suggested that APP acts as a signaling nexus that transduces information about a range of extracellular conditions, including neuronal damage, to induction of intracellular signaling events and may be major contributors to AD-causing neuronal dysfunction.

TL;DR: It is proposed that F-actin turnover is the driving force in myelin wrapping by regulating repetitive cycles of leading edge protrusion and spreading.

TL;DR: It is proposed that IFT27 separates from IFT-B inside cilia to promote ARL6 activation, BBSome coat assembly, and subsequent ciliary exit, mirroring the process by which BBSome mediates cargo entry into cilia.

TL;DR: It is found that most protein levels change little and duplicated genes are expressed similarly, and a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration.

TL;DR: It is demonstrated that ERG is an essential regulator of angiogenesis and vascular stability through Wnt signaling through signals mediated by VE-cadherin and the Wnt receptor Frizzled-4.

TL;DR: It is demonstrated that depletion of CALM causes a substantial increase in the ratio of “open” clathrin-coated pits (CCPs) to “necked”/“closed” CCVs and a doubling of CCP/CCV diameter, whereas AP2 depletion has opposite effects.

TL;DR: A framework for understanding neural PCD from its regulation to its functions is provided and the underlying PCD molecular mechanisms include intrinsic transcription factor cascades and regulators of competence/susceptibility to cell death.