Steffi De Rouck

TL;DR: The utility of HuRI is demonstrated in identifying the specific subcellular roles of protein–protein interactions and in identifying potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases.

TL;DR: The first human “all-by-all” binary reference interactome map, or HuRI, is presented and it is demonstrated the use of HuRI in identifying specific subcellular roles of PPIs and protein function modulation via splicing during brain development.

TL;DR: This project applied state-of-the-art experimental and bioinformatics methods to identify high-confidence binary protein-protein interactions (PPIs) for Drosophila melanogaster, and used novel interactions from the FlyBi dataset to generate an autophagy interaction network that was validated in vivo using two different autophile-related assays.

TL;DR: The RNA-KISS method is validated as a robust and high-throughput cell-based RNA-protein interaction screening tool for proteome-wide screening of flavivirus host factors and confirms the previously established interaction between the RNA-binding domain of DDX6 and the DENV sfRNA.