Gloria M. Sheynkman

TL;DR: The utility of HuRI is demonstrated in identifying the specific subcellular roles of protein–protein interactions and in identifying potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases.

TL;DR: This work cloned full-length open reading frames of alternatively spliced transcripts for a large number of human genes and used protein-protein interaction profiling to functionally compare hundreds of protein isoform pairs, revealing a widespread expansion of protein interaction capabilities through alternative splicing and suggesting that many alternative "isoforms" are functionally divergent (i.e., "functional alloforms").

TL;DR: The knowledge gained from these and complementary studies provides fresh insights into the opportunities and challenges when analyzing systematically generated interactome data, defines a clear roadmap towards the generation of a first reference interactome, and reveals new perspectives on the organization of cellular life.

TL;DR: This is the first example of using sample-specific RNA-Seq data to create a splice-junction database and discover new peptides resulting from alternative splicing, and a bioinformatics workflow to retrieve high-confidence, novel splice junction sequences from the RNA data, translate these sequences into the analogous polypeptide sequence, and create a customizedsplice junction database for MS searching.

TL;DR: The field of human proteogenomics is reviewed, with an emphasis on its history, current implementations, the types of proteomic variations it reveals, and several important applications.