Sudharshan Rangarajan

TL;DR: The utility of HuRI is demonstrated in identifying the specific subcellular roles of protein–protein interactions and in identifying potential molecular mechanisms that might underlie tissue-specific phenotypes of Mendelian diseases.

TL;DR: The authors show that complete coverage could be reached with a minimal number of assays as long as they explore various experimental conditions, and suggest that developing alternative versions of existing assays might be as productive as designing completely new assays.

TL;DR: The first human “all-by-all” binary reference interactome map, or HuRI, is presented and it is demonstrated the use of HuRI in identifying specific subcellular roles of PPIs and protein function modulation via splicing during brain development.

TL;DR: The concept of an “assayome” for binary protein-protein interaction (PPI) mapping is proposed as an optimal combination of assays and/or assay versions that maximizes detection of true positive interactions, while avoiding detection of random protein pairs.

TL;DR: A framework for binary protein-protein interaction (PPI) mapping based on optimally combining assays and/or assay versions to maximize detection of true positive interactions, while avoiding detection of random protein pairs is proposed.