S
Stephen J. Tucker
Researcher at University of Oxford
Publications - 158
Citations - 9006
Stephen J. Tucker is an academic researcher from University of Oxford. The author has contributed to research in topics: Gating & Potassium channel. The author has an hindex of 47, co-authored 143 publications receiving 8165 citations. Previous affiliations of Stephen J. Tucker include Kyoto University & Pontifical Catholic University of Chile.
Papers
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Journal ArticleDOI
Inward rectification in KATP channels: a pH switch in the pore
TL;DR: It is shown that in cardiac as well as in cloned KATP channels (Kir6.2 + sulfonylurea receptor) polyamine‐mediated rectification is not fixed but changes with intracellular pH in the physiological range: inward‐rectification is prominent at basic pH, while at acidic pH rectifying is very weak.
Journal ArticleDOI
A pharmacological master key mechanism that unlocks the selectivity filter gate in K+ channels.
Marcus Schewe,Han Sun,Ümit Mert,Alexandra Mackenzie,Alexandra Mackenzie,Ashley C. W. Pike,Friederike Schulz,Cristina E. Constantin,Kirsty S Vowinkel,Linus J. Conrad,Aytug K. Kiper,Wendy González,Marianne Musinszki,Marie Tegtmeier,David C. Pryde,Belabed Hassane,Marc Nazaré,Bert L. de Groot,Niels Decher,Bernd Fakler,Elisabeth P. Carpenter,Elisabeth P. Carpenter,Stephen J. Tucker,Thomas Baukrowitz +23 more
TL;DR: An unrecognized polypharmacology among K+ channel activators is uncovered and a filter gating machinery that is conserved across different families of K+ channels with implications for rational drug design is highlighted.
Journal ArticleDOI
Ion mobility mass spectrometry of two tetrameric membrane protein complexes reveals compact structures and differences in stability and packing.
Sheila C. Wang,Argyris Politis,Natalie D. Di Bartolo,Vassiliy N. Bavro,Stephen J. Tucker,Paula J. Booth,Nelson P. Barrera,Carol V. Robinson +7 more
TL;DR: The collision cross sections measured for the ion channel are in accord with a compact configuration of subunits, suggesting that the native-like structure can be preserved under the harsh activation conditions required to release it from the detergent micelle into the gas phase.
Journal ArticleDOI
Identification and developmental expression of the Xenopus laevis cystic fibrosis transmembrane conductance regulator gene
TL;DR: Investigation of the expression of the Xenopus gene during early stages of development, using RNAase protection assays and PCR analysis of total Xenopus RNA, shows CFTR mRNA to be present at the very earliest stages ofDevelopment, including the oocyte and blastula stages, with increasing amounts during subsequent development.
Journal ArticleDOI
H Bonding at the Helix-Bundle Crossing Controls Gating in Kir Potassium Channels
Markus Rapedius,Philip W. Fowler,Lijun Shang,Mark S.P. Sansom,Stephen J. Tucker,Thomas Baukrowitz +5 more
TL;DR: It is demonstrated that H+ and PIP2 use a conserved gating mechanism defined by similar structural changes in the transmembrane (TM) helices and the selectivity filter, which defines a common gating motif in the Kir channel superfamily.