T
Thomas S. Walter
Researcher at Wellcome Trust Centre for Human Genetics
Publications - 21
Citations - 1622
Thomas S. Walter is an academic researcher from Wellcome Trust Centre for Human Genetics. The author has contributed to research in topics: Crystallization & RANKL. The author has an hindex of 14, co-authored 21 publications receiving 1473 citations. Previous affiliations of Thomas S. Walter include University of Oxford.
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Journal ArticleDOI
Lysine methylation as a routine rescue strategy for protein crystallization.
Thomas S. Walter,Christoph Meier,Rene Assenberg,Kin Fai Au,Jingshan Ren,Anil Verma,Joanne E. Nettleship,Raymond J. Owens,David I. Stuart,Jonathan M. Grimes +9 more
TL;DR: In this paper, the reductive methylation of lysine residues was used to obtain diffraction-quality crystals from four proteins and structures for three that had previously proved refractory to crystallization.
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Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy
Janet Newman,D. Egan,Thomas S. Walter,Ran Meged,I M Berry,M. Ben Jelloul,Joel L. Sussman,David I. Stuart,Anastassis Perrakis +8 more
TL;DR: The systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space.
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A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization.
Thomas S. Walter,J.M. Diprose,C J Mayo,Christian Siebold,M.G. Pickford,Lester G. Carter,Geoff Sutton,Nick S. Berrow,J. Brown,I M Berry,Guillaume Stewart-Jones,Jonathan M. Grimes,David K. Stammers,Robert Esnouf,E Y Jones,Raymond J. Owens,David I. Stuart,Karl Harlos +17 more
TL;DR: Crystallization trials at the Division of Structural Biology in Oxford are now almost exclusively carried out using a high‐throughput workflow implemented in the Oxford Protein Production Facility, which includes a protocol for variation of pH, reservoir dilution and protein:reservoir ratio and an additive screen.
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Picornavirus uncoating intermediate captured in atomic detail
Jingshan Ren,Xiangxi Wang,Zhongyu Hu,Qiang Gao,Yao Sun,Xuemei Li,Claudine Porta,Thomas S. Walter,Robert J.C. Gilbert,Yuguang Zhao,Danny Axford,Mark Williams,Katherine McAuley,David J. Rowlands,Weidong Yin,Junzhi Wang,David I. Stuart,Zihe Rao,Zihe Rao,Zihe Rao,Elizabeth E. Fry +20 more
TL;DR: The atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16 is presented, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane.
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In situ macromolecular crystallography using microbeams.
Danny Axford,Robin L. Owen,Jun Aishima,James Foadi,Ann W. Morgan,James I. Robinson,Joanne E. Nettleship,Raymond J. Owens,Isabel Moraes,Elizabeth E. Fry,Jonathan M. Grimes,Karl Harlos,Abhay Kotecha,Jingshan Ren,Geoff Sutton,Thomas S. Walter,David I. Stuart,Gwyndaf Evans +17 more
TL;DR: A sample environment for mounting crystallization trays has been developed on the microfocus beamline I24 at Diamond Light Source and several case studies are described.