R
Raymond J. Owens
Researcher at University of Oxford
Publications - 234
Citations - 12349
Raymond J. Owens is an academic researcher from University of Oxford. The author has contributed to research in topics: Antibody & Binding site. The author has an hindex of 58, co-authored 225 publications receiving 10643 citations. Previous affiliations of Raymond J. Owens include Rutherford Appleton Laboratory & University of Sheffield.
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Journal ArticleDOI
Protein production and purification.
Susanne Gräslund,Pär Nordlund,J Weigelt,B M Hallberg,James E. Bray,Opher Gileadi,Stefan Knapp,Udo Oppermann,Cheryl H. Arrowsmith,Raymond Hui,J Ming,Sirano Dhe-Paganon,Park H-W.,Alexei Savchenko,Adelinda Yee,Aled M. Edwards,Renaud Vincentelli,Christian Cambillau,R Kim,Kim S-H.,Zihe Rao,Yunyu Shi,Thomas C. Terwilliger,Kim C-Y.,Hung L-W.,Geoffrey S. Waldo,Yoav Peleg,Shira Albeck,Tamar Unger,Orly Dym,Jaime Prilusky,Joel L. Sussman,Raymond C. Stevens,Scott A. Lesley,Scott A. Lesley,Ian A. Wilson,Ian A. Wilson,Andrzej Joachimiak,Frank R. Collart,Irina Dementieva,Mark I. Donnelly,William H. Eschenfeldt,Youngchang Kim,Lucy Stols,Ruiying Wu,M. Zhou,Stephen K. Burley,J.S. Emtage,J.M. Sauder,D.A. Thompson,Kevin Bain,John G. Luz,Tarun Gheyi,F Zhang,Shane Atwell,Steven C. Almo,Jeffrey B. Bonanno,Andras Fiser,Soumya Swaminathan,F.W. Studier,Mark R. Chance,Andrej Sali,Thomas Acton,Rong Xiao,L Zhao,L C Ma,John F. Hunt,Liang Tong,Kellie Cunningham,Masayori Inouye,Stephen Anderson,Haleema Janjua,Ritu Shastry,C K Ho,D Wang,Hao Wang,M Jiang,Gaetano T. Montelione,David I. Stuart,Raymond J. Owens,S Daenke,Anja Schütz,Udo Heinemann,S Yokoyama,Konrad Büssow,Kristin C. Gunsalus,Kristin C. Gunsalus +86 more
TL;DR: This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators.
Journal ArticleDOI
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
Nick S. Berrow,David Alderton,Sarah Sainsbury,Joanne E. Nettleship,Rene Assenberg,Nahid Rahman,David I. Stuart,Raymond J. Owens +7 more
TL;DR: The construction of a set of versatile expression vectors based on the In-Fusion™ cloning enzyme and their use for high-throughput cloning and expression screening and the development and validation of automated protocols for transfection of mammalian cells and Ni-NTA protein purification.
Journal ArticleDOI
Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease
Nathan R. West,Ahmed N. Hegazy,Owens Bmj.,Samuel J. Bullers,Bryan Linggi,Sofia Buonocore,Margherita Coccia,Dieter Görtz,S This,K Stockenhuber,Johanna Pott,Matthias Friedrich,Grigory Ryzhakov,Frédéric Baribaud,Carrie Brodmerkel,C Cieluch,Nahid Rahman,Gerhard Müller-Newen,Raymond J. Owens,Raymond J. Owens,Anja A. Kühl,Kevin J. Maloy,S Plevy,Satish Keshav,Travis Spl.,Fiona Powrie +25 more
TL;DR: It is shown that, relative to healthy controls, inflamed intestinal tissues from patients with IBD express high amounts of the cytokine oncostatin M (OSM) and its receptor (OSMR), which correlate closely with histopathological disease severity.
Journal ArticleDOI
Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2.
J. Huo,J. Huo,A. Le Bas,A. Le Bas,Reinis R. Ruza,Duyvesteyn Hme.,H. Mikolajek,Tomas Malinauskas,Tiong Kit Tan,Pramila Rijal,Pramila Rijal,Maud Dumoux,Philip N. Ward,Philip N. Ward,Jingshan Ren,D. Zhou,Peter J. Harrison,Peter J. Harrison,Miriam Weckener,Daniel K. Clare,V K Vogirala,Julika Radecke,Lucile Moynié,Yuguang Zhao,Javier Gilbert-Jaramillo,Michael L. Knight,Julia A. Tree,Karen R. Buttigieg,Naomi Coombes,Michael J. Elmore,Miles W. Carroll,Loic Carrique,Shah Pnm.,William James,Alain Townsend,Alain Townsend,David I. Stuart,Raymond J. Owens,Raymond J. Owens,James H. Naismith,James H. Naismith +40 more
TL;DR: Two nanobodies that bind SARS-CoV-2 spike RBD are shown to block interaction with receptor ACE2 and thus neutralize the virus, and have an additive effect with antibody CR3022.
Journal ArticleDOI
Neutralization of SARS-CoV-2 by Destruction of the Prefusion Spike.
Jiandong Huo,Jiandong Huo,Yuguang Zhao,Jingshan Ren,D. Zhou,Helen M. E. Duyvesteyn,Helen M. Ginn,Loic Carrique,Tomas Malinauskas,Reinis R. Ruza,Pranav N.M. Shah,Tiong Kit Tan,Pramila Rijal,Naomi Coombes,Kevin R. Bewley,Julia A. Tree,Julika Radecke,Neil G. Paterson,P Supasa,Juthathip Mongkolsapaya,Juthathip Mongkolsapaya,Gavin R. Screaton,Miles W. Carroll,Miles W. Carroll,Alain Townsend,Elizabeth E. Fry,Raymond J. Owens,Raymond J. Owens,David I. Stuart +28 more
TL;DR: It is found that the monoclonal antibody CR3022 binds the RBD tightly, neutralising SARS-CoV-2 and the crystal structure at 2.4 Å of the Fab/RBD complex is reported, suggesting that CR30 22 binding facilitates conversion to the fusion-incompetent post-fusion state.