Michael Ratz

TL;DR: This work shows that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves, providing isotropic resolution in the focal plane and making pattern rotation redundant.

TL;DR: CRISPR/Cas9-mediated generation of heterozygous and homozygous human knockin cell lines expressing fluorescently tagged proteins from their respective native genomic loci at close to endogenous levels is established and can be extended to other fluorescent tags and super-resolution approaches.

TL;DR: STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost is presented, matching the performance of a previous lower-throughput platform while retaining a high degree of flexibility.

TL;DR: This article examined the transcriptional states of neural crest-and mesoderm-derived lineages differentiating into adrenal glands, kidneys, endothelium and hematopoietic tissue between post-conception weeks 6 and 14 of human development.

TL;DR: Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.