Timothy Mosbruger

TL;DR: The results demonstrate the potential of nanopore sequencing for delivering accurate HR-3F typing with a simple, rapid, and cost-effective protocol and appears to offer a significant advancement over current next-generation sequencing platforms as a single solution for all HLA genotyping needs.

TL;DR: The role of high‐resolution 2‐field HLA typing (HR‐2F) in SOT is assessed by retrospectively evaluating NGS‐typed pre‐ and post‐SOT cases, whereby 21% of the cases required HR‐ 2F typing by Sanger sequencing, as supported by other legacy methods, to properly address posttransplant anti‐HLA antibody issues.

TL;DR: The authors' approach of multiple-laboratory NGS HLA typing of the B-LCLs has provided accurate genotyping data and is useful for updating the current cell and sequence databases and will be a valuable resource for future studies.

TL;DR: Oxford Nanopore, a third generation sequencing technology, was used to resolve the phasing of exonic heterozygous positions between exon 2 and all other downstream exons and the accurate MiSeq sequencing data combined with the long reads obtained from the MinION platform of Nanobore technology allows for the resolution of the tested ambiguities.

TL;DR: The goal was to develop a publicly available and renewable source of well-characterized genomic DNA reference materials to support molecular HLA typing assay development, validation, and verification, quality control, and proficiency testing.