Assessing the utilization of high‐resolution 2‐field HLA typing in solid organ transplantation
Yanping Huang,Anh Dinh,S. Heron,Allison Gasiewski,Carolina Kneib,Hilary Mehler,Michael T. Mignogno,Ryan Morlen,Larissa Slavich,Ethan Kentzel,Edward C. Frackelton,Jamie L. Duke,Deborah Ferriola,Timothy Mosbruger,Olga A. Timofeeva,Steven S. Geier,Dimitri S. Monos,Dimitri S. Monos +17 more
TLDR
The role of high‐resolution 2‐field HLA typing (HR‐2F) in SOT is assessed by retrospectively evaluating NGS‐typed pre‐ and post‐SOT cases, whereby 21% of the cases required HR‐ 2F typing by Sanger sequencing, as supported by other legacy methods, to properly address posttransplant anti‐HLA antibody issues.About:
This article is published in American Journal of Transplantation.The article was published on 2019-02-22 and is currently open access. It has received 32 citations till now. The article focuses on the topics: Typing.read more
Citations
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Journal ArticleDOI
Clinical importance of extended second field high‐resolution HLA genotyping for kidney transplantation
Aleksandar Senev,Marie-Paule Emonds,Vicky Van Sandt,Evelyne Lerut,Maarten Coemans,Ben Sprangers,Dirk Kuypers,Maarten Naesens +7 more
TL;DR: It is concluded that extended 2F‐HR typing of the donor‐recipient pairs is relevant for the correct assessment of DSA, and significant misclassification occurs, and warrants caution in using inferred HLA results for clinical and research purposes.
Journal ArticleDOI
Utilizing nanopore sequencing technology for the rapid and comprehensive characterization of eleven HLA loci; addressing the need for deceased donor expedited HLA typing.
Timothy Mosbruger,Amalia Dinou,Jamie L. Duke,Deborah Ferriola,Hilary Mehler,Ioanna Pagkrati,Georgios Damianos,Eric Mbunwe,Mahdi Sarmady,Ioannis Lyratzakis,Sarah A. Tishkoff,Anh Dinh,Dimitri S. Monos,Dimitri S. Monos +13 more
TL;DR: The results demonstrate the potential of nanopore sequencing for delivering accurate HR-3F typing with a simple, rapid, and cost-effective protocol and appears to offer a significant advancement over current next-generation sequencing platforms as a single solution for all HLA genotyping needs.
Journal ArticleDOI
Comparison of sequence-specific oligonucleotide probe vs next generation sequencing for HLA-A, B, C, DRB1, DRB3/B4/B5, DQA1, DQB1, DPA1, and DPB1 typing: Toward single-pass high-resolution HLA typing in support of solid organ and hematopoietic cell transplant programs.
Anajane G. Smith,Shalini Pereira,Andrés Jaramillo,Scott T. Stoll,Faisal M. Khan,Noureddine Berka,Ahmed A. Mostafa,Marcelo J. Pando,Crystal Y. Usenko,Maria Bettinotti,Chul Woo Pyo,Wyatt C. Nelson,Amanda Willis,Medhat Askar,Daniel E. Geraghty +14 more
TL;DR: This study provides compelling evidence that, although not viable for STAT typing of deceased donors, a single‐pass NGS HLA typing method has direct application for solid organ transplantation.
Journal ArticleDOI
A long road/read to rapid high-resolution HLA typing: The nanopore perspective.
TL;DR: An overview of next-generation sequencing and the opportunities and challenges of using nanopore sequencing for high-resolution HLA typing and immunogenetics research are focused on.
Journal ArticleDOI
Performance of a multiplexed amplicon-based next-generation sequencing assay for HLA typing.
Chang Liu,Brian Duffy,Eric T. Weimer,Eric T. Weimer,Maureen C. Montgomery,Jo Ellen Jennemann,Rachel Hill,Donna Phelan,Lindsay Lay,Bijal A. Parikh +9 more
TL;DR: The AllType assay on the Ion Chef/Ion S5 platform offers a robust and efficient workflow for clinical HLA typing at the 2-field resolution and the multiplex PCR strategy simplifies the laboratory procedure without compromising the typing accuracy.
References
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Class II HLA epitope matching-A strategy to minimize de novo donor-specific antibody development and improve outcomes.
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TL;DR: HLA‐DR and DQ epitope matching outperforms traditional low‐resolution antigen‐based matching and has the potential to minimize the risk of de novo Class II DSA development, thereby improving long‐term graft outcome.
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