V
Vitaly Polovinkin
Researcher at Academy of Sciences of the Czech Republic
Publications - 31
Citations - 987
Vitaly Polovinkin is an academic researcher from Academy of Sciences of the Czech Republic. The author has contributed to research in topics: Rhodopsin & Integral membrane protein. The author has an hindex of 14, co-authored 31 publications receiving 743 citations. Previous affiliations of Vitaly Polovinkin include Forschungszentrum Jülich & Moscow Institute of Physics and Technology.
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Journal ArticleDOI
Structural insights into ion conduction by channelrhodopsin 2
Oleksandr Volkov,Kirill Kovalev,Vitaly Polovinkin,Valentin Borshchevskiy,Christian Bamann,R. Astashkin,R. Astashkin,Egor Marin,Alexander Popov,Taras Balandin,Dieter Willbold,Dieter Willbold,Georg Büldt,Ernst Bamberg,Valentin Gordeliy,Valentin Gordeliy +15 more
TL;DR: The high-resolution structure of channelrhodopsin 2, the most widely used optogenetics tool, as well as the structure of a mutant with a longer open-state lifetime are described, providing a basis for designing better optogenetic tools.
Journal ArticleDOI
Mechanism of transmembrane signaling by sensor histidine kinases
Ivan Gushchin,Igor Melnikov,Vitaly Polovinkin,Vitaly Polovinkin,Andrii Ishchenko,Anastasia Yuzhakova,Pavel Buslaev,Gleb Bourenkov,Sergei Grudinin,Sergei Grudinin,Sergei Grudinin,Ekaterina Round,Taras Balandin,Valentin Borshchevskiy,Dieter Willbold,Gordon A. Leonard,Georg Büldt,Alexander Popov,Valentin Gordeliy,Valentin Gordeliy +19 more
TL;DR: The structures reveal the mechanism of transmembrane signal transduction in NarQ and show that binding of ligand induces displacement of the sensor domain helices by ~0.5 to 1 Å and demonstrate that the signaling-associated conformational changes in the TM domain do not need to be symmetric.
Journal ArticleDOI
Crystal structure of a light-driven sodium pump
Ivan Gushchin,Vitaly Shevchenko,Vitaly Shevchenko,Vitaly Polovinkin,Kirill Kovalev,Alexey Alekseev,Ekaterina Round,Valentin Borshchevskiy,Taras Balandin,Alexander Popov,Thomas Gensch,Christoph Fahlke,Christian Bamann,Dieter Willbold,Georg Büldt,Ernst Bamberg,Valentin Gordeliy +16 more
TL;DR: The structures of Krokinobacter eikastus rhodopsin 2 show that the sodium ion is bound outside the protein at the oligomerization interface, that the ion-release cavity is capped by a unique N-terminal α-helix and that the Ion-uptake cavity is unexpectedly large and open to the surface.
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Inward H$^+$ pump xenorhodopsin: Mechanism and alternative optogenetic approach
Vitaly Shevchenko,Vitaly Shevchenko,Thomas Mager,Kirill Kovalev,Kirill Kovalev,Vitaly Polovinkin,Vitaly Polovinkin,Alexey Alekseev,Alexey Alekseev,Josephine Juettner,Igor Chizhov,Christian Bamann,Charlotte D. Vavourakis,Rohit Ghai,Ivan Gushchin,Valentin Borshchevskiy,A. V. Rogachev,A. V. Rogachev,Igor Melnikov,Alexander Popov,Taras Balandin,Francisco Rodriguez-Valera,Dietmar J. Manstein,G. Bueldt,Ernst Bamberg,Valentin Gordeliy,Valentin Gordeliy +26 more
TL;DR: It is demonstrated that the NsXeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency, and they are an alternative to the well-known cation-selective channelrhodopsins.
Journal ArticleDOI
Structure and mechanisms of sodium-pumping KR2 rhodopsin.
Kirill Kovalev,Vitaly Polovinkin,Ivan Gushchin,Alexey Alekseev,Alexey Alekseev,Vitaly Shevchenko,Vitaly Shevchenko,Valentin Borshchevskiy,R. Astashkin,R. Astashkin,Taras Balandin,Dmitry Bratanov,Svetlana Vaganova,Alexander Popov,Vladimir Chupin,Georg Büldt,Ernst Bamberg,Ernst Bamberg,Valentin Gordeliy,Valentin Gordeliy +19 more
TL;DR: The structure of the cationic (non H+) light-driven pump at physiological pH in its pentameric form provides new insights into the mechanisms of microbial rhodopsins and opens the way to a rational design of novel cation pumps for optogenetics.