Yuko Fujioka

TL;DR: Results indicate that the Atg12-Atg5 conjugate is a ubiquitin-protein ligase (E3)-like enzyme for Atg8-PE conjugation reaction, distinctively promoting protein-lipid conjugations.

TL;DR: The results show the fundamental mechanism that allows Atg8 homologues, in association with WXXL‐containing proteins, to capture specific cargo molecules, thereby endowing isolation membranes and/or their assembly machineries with target selectivity.

TL;DR: The crystal structures of catalytically inert human Atg4 B (HsAtg4B) in complex with processed and unprocessed forms of LC3, a mammalian orthologue of yeast Atg8, are reported.

TL;DR: In vitro experiments show that Atg1-complex droplets can be tethered to membranes via specific protein–protein interactions, explaining the vacuolar membrane localization of the PAS in vivo, and propose that phase separation has a critical, active role in autophagy, whereby it organizes the autophagic machinery at the P AS.

TL;DR: It is proposed that Ypk protein kinases are direct and essential targets ofTORC2, coupling TORC2 to the cell integrity cascade.