Zhengjing Zhang

TL;DR: The work demonstrates that the CRISPR/Cas method can effectively create specific gene modifications in planta that are stably transmitted through the germ line to future generations and is a useful tool for generating versatile and heritable modifications specifically at target genes in plants.

TL;DR: The results not only demonstrate that the three CBF genes together are required for cold acclimation and freezing tolerance, but also reveal that they are important for salt tolerance and seedling development.

TL;DR: The design and characterization of a germ-line-specific Cas9 system (GSC) for Arabidopsis gene modification in male gametocytes is reported, constructed using a SPOROCYTELESS genomic expression cassette, and the results suggest that future application of the described GSC system will facilitate the screening for targeted gene modifications, especially lethal mutations in the T2 population.

TL;DR: A new multiplex CRISPR/Cas9 system that allows the co-expression of six sgRNA modules in one binary vector using a simple cloning strategy in Arabidopsis is designed and it is anticipated that the application of this multiplex system will strongly facilitate functional analysis of genes pathways and families.

TL;DR: The results demonstrate an important role of basal ABA signaling in growth, senescence, and abscission and reveal that PYLs antagonize ABA-independent activation of SnRK2s by osmotic stress.