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Showing papers by "California State University, Long Beach published in 1986"


Journal ArticleDOI
TL;DR: The Activation-Deactivation Adjective Check List (AD ACL) as discussed by the authors is a multidimensional self-rating test constructed and extensively validated for rapid assessments of momentary activation or arousal state.
Abstract: The Activation-Deactivation Adjective Check List (AD ACL) is a multidimensional self-rating test constructed and extensively validated for rapid assessments of momentary activation or arousal state...

396 citations


Journal ArticleDOI
TL;DR: Young students, old students, and old nonstudents read and recalled short texts that were in either narrative or expository form and a set of six verbal ability measures thought to be related to discourse memory was obtained.
Abstract: Young students, old students, and old nonstudents read and recalled short texts that were in either narrative or expository form. In addition, a set of six verbal ability measures thought to be related to discourse memory was obtained for all of the participants. Older subjects recalled less from the texts than younger subjects, and neither type of text nor student status modified the magnitude of the age differences. The set of verbal ability measures was adequately described by two principal components, one consisting of simple, speed-related skills, and the other consisting of more complex skills. Each set of variables predicted a proportion of discourse memory variance, and accounted for some of the variance that would otherwise be attributed to age. The results suggest that age differences in some basic cognitive skills related to reading effectiveness might underlie age differences in memory for discourse.

104 citations


Journal ArticleDOI
TL;DR: In this article, a viscous/inviscid interaction method is described and has been used to calculate flows around four distinctly different airfoils as a function of angle of attack.
Abstract: A viscous/inviscid interaction method is described and has been used to calculate flows around four distinctly different airfoils as a function of angle of attack. It comprises an inviscid-flow method based on conformal mapping, a boundary-layer procedure based on the numerical solution of differential equations and an algebraic eddy viscosity. The results are in close agreement with experiment up to angles close to stall. In one case, where the airfoil thickness is large, small difficulties were experienced and are described. The method is shown to be capable of obtaining results with large flow separation and quantifies the role of transition on the lift coefficient.

102 citations


Journal ArticleDOI
TL;DR: In this article, a heuristic is developed based on the incremental cost of increasing the lot size by one unit, which minimizes the total of production, setup, holding, shortage, and scrap costs.
Abstract: Consider a job shop which must completely fill a large make-to-order demand of a product where production yield is highly variable. After a production lot is completed, if the total output of satisfactory units is inadequate to satisfy the demand, then a new run (with associated setup cost) is made. When the output of good units exceeds the demand, then the excess units are scrapped (with possible salvage value). The optimal lot size minimizes the total of production, setup, holding, shortage, and scrap costs. A heuristic is developed based on the incremental cost of increasing the lot size by one unit. The computational ease and excellent cost performance of the heuristic favor its use in place of the mathematically optimal solution obtained by dynamic programming. Real world manufacturing applications and additional properties of the model are also discussed.

71 citations


Journal ArticleDOI
TL;DR: Pharmacist-managed warfarin therapy in these clinic patients resulted in a level of anticoagulation control and morbidity that was acceptable to physicians.
Abstract: A pharmacist-managed anticoagulation clinic is described, and information on patient outcome during a nine-year period is presented. Since 1974, a pharmacist has managed an anticoagulation clinic for ambulatory patients and inpatients at San Francisco General Hospital Medical Center. The pharmacist's primary responsibilities include the following: educating patients about their diseases and the importance of drug therapy, monitoring patients' vital signs, performing physical examinations, and adjusting warfarin dosage to maintain prothrombin times within the therapeutic range (1.7-2.5 times normal using control values of 1.0-1.2). These patients are also under the care of their primary physicians. The pharmacist's work is checked by the chief of the cardiac clinic at the end of each clinic session. The effectiveness of the pharmacist in managing clinic patients is reviewed periodically; from January 1975 through June 1984, the pharmacist had treated 140 patients (141 courses of therapy). Of 1792 prothrombin times taken during this time, 1060 (59.2%) were within the therapeutic range of 17-25 seconds, 510 (28.5%) were less than 17 seconds, and 222 (12.4%) were greater than 25 seconds. Only four major hemorrhagic events (0.002 hemorrhages per patient-treatment month) and 89 minor events (0.05 hemorrhages per patient-treatment month) occurred. The recurrence rate of thromboembolic events was 0.007 per patient-treatment month. Pharmacist-managed warfarin therapy in these clinic patients resulted in a level of anticoagulation control and morbidity that was acceptable to physicians.

68 citations


Journal ArticleDOI
TL;DR: This account describes the comparative response of four species of benthic invertebrates to burial in terms of vertical migration and mortality, and provides a synthesis of studies and recommendations upon which to assess future impacts.
Abstract: This account describes the comparative response of four species of benthic invertebrates to burial in terms of vertical migration and mortality, and provides a synthesis of studies and recommendations upon which to assess future impacts. The species featured were the bivalve Mercenaries mercenaria, the amphipod crustacean Parahaustorius longimerus, and the polychaetes Scoloplos fragilis and Nereis succinea. There was evidence of synergistic effects on burrowing activity and mortality with changes in time of burial, sediment depth, sediment type and temperature. In sediment with silt-clay, N. succinea was the most resistant species followed by M. mercenaria, S. fragilis and P. longimerus. In sediment without silt-clay the order of percent mortality was reversed. Studies of surface water chemistry and sediment geochemistry showed that dissolved oxygen decreased significantly and ammonia and sulfide increased significantly between the surface and below 2.0 cm within a 15-day period. Based on these results and physiological tolerances from the literature it was concluded that M. mercenaria and N. succinea would be relatively resistant to chemical effects of spoil disposal, whereas S. fragilis and P. longimerus would be less resistant to such effects. Vertical migration of benthic invertebrates through dredge disposal can be a viable mechanism of recolonization under certain conditions. Some effects of burial of benthos can be predicted based on morphology, behavior and physiology. These biological features were discussed with examples dealing with molluscs, crustaceans, and polychaetes. Finally, recommendations were made concerning the type of studies to provide additional data to aid management agencies in decision making about future dredging and disposal practices.

58 citations


Patent
20 Mar 1986
TL;DR: In this paper, a stationary material mixing apparatus for mixing various components in a fluid stream is described, which is in the shape of a conduit which is made up of individual biscuit sections which are aligned along a longitudinal axis, each containing a plurality of openings there through where within openings are located mixing elements which induce a rotational angular velocity to the fluid stream.
Abstract: A stationary material mixing apparatus for mixing various components in a fluid stream. The mixing apparatus is in the shape of a conduit which is made up of individual biscuit sections which are aligned along a longitudinal axis, the biscuit sections each containing a plurality of openings therethrough where within openings are located mixing elements which induce a rotational angular velocity to the fluid stream.

53 citations


Journal ArticleDOI
TL;DR: In this article, a growth of the nonsulfate-reducing marine bacterium Vibrio natriegens increased the corrosion current density (CD) of AISI 304 stainless steel (SS) coupons when grown in a marine medium.
Abstract: Growth of the nonsulfate-reducing marine bacterium Vibrio natriegens increased the corrosion current density (CD) of AISI 304 stainless steel (SS) coupons when grown in a marine medium. The corrosion rate, estimated as the corrosion CD (Icorr), calculated from the Tafel constants and polarization resistance, increased from 230 to 2900 nA/cm2 during a 6-day incubation period with V. natriegens. Just before the rapid increase in the corrosion CD, bacterial cells were seen by epifluorescent microscopy after acridine orange staining to colonize the SS surface. On the third day of exposure to seawater, the rapid increase in the corrosion CD correlated with the appearance of extracellular material from the colonizing bacteria, as observed by (1) epifluorescent microscopy, (2) scanning electron microscopy (SEM), and (3) nondestructive analysis of lyophilized biofilms by Fourier transform-infrared spectroscopy (FT-IR) using diffuse reflectance. The extracellular products from the colonizing bacteria corr...

53 citations


Journal ArticleDOI
TL;DR: Although Cebidichthys violaceus and Xiphister mucosus seem to be able to grow during most times of the year on a completely algal diet, their rates of energy consumption, which in turn regulate protein intake, are unknown and must be examined before more definite conclusions can be drawn about the growth of these fishes.

52 citations


Patent
21 Jan 1986
TL;DR: A self-coupling split-shaft drive assembly for a tool provides for assembly by a user and start-up without concern over the proper or improper orientation of the two shafts.
Abstract: A self-coupling split-shaft drive assembly for a tool provides for assembly by a user and start-up without concern over the proper or improper orientation of the two shafts. The drive assembly includes components to secure the two shafts for start-up and use independently of whether an orientation condition exists for their rotational coupling to one another. A shaft coupling mechanism then accomplishes the coupling where an improper condition exists after the securing, in response to the initiation of the rotation of the driver shaft by the motor. The coupling mechanism employs a driver shaft which, with a shaft socket, for receiving the driven shaft, slides back and forth, against a spring and then under the recoil force of the spring, in providing for the securing under an improper orientation condition and the subsequent coupling upon start-up. A split clamp, which is used on a driver shaft tube and driven shaft tube is particularly adapted for rapid, easily accomplished and, at the same time, extremely sturdy clamping of elongated members such as the shaft tubes. The clamp employs a pair of clamp member which are formed, with respect to the elongated members, to a designed clamp face separation of less than or equal to about 3.4 percent and greater than or equal to about 0.5 percent of the diameter for the elongated members, among other significant features. Manually tightened and loosened connectors for one end of the clamp members and tool-operated tightening and loosening at the other end signifcantly contribute to the indicated goals.

49 citations


Journal ArticleDOI
TL;DR: Some of the methods that minimize the risks to the mother, fetus, and neonate in postdate pregnancy are outlined and a synopsis of research areas that may change management is given.

Journal ArticleDOI
TL;DR: The activities of blacksmiths may results in the importation of extrinsic, inorganic nitrogen to primary producers on temperate Reefs, off southern California, USA, at night.
Abstract: The blacksmith Chromis punctipinnis, an abundant planktivorous damselfish off southern California, USA, shelters along rocky reefs at night. While sheltered, blacksmiths excrete ammonium that could, in turn, be utilized by nearby benthic macrophytes. Laboratory experiments during the summer and fall of 1983 and 1984 indicate that ammonium excretion at night ranged from 18.1 μmol h-1 by a 8.5 g (dry) fish, to 89.1 μmol h-1 by a 27.3 g fish; excretion rates generally declined throughout the night. Field measurements at night indicate that ammonium concentrations were significantly higher in rocky crevices occupied by blacksmiths than in unoccupied shelters, and the ammonium level in one shelter dropped after a blacksmith was experimentally removed. Young kelp plants (Macrocystis pyrifera) are capable of taking up ammonium at night. Ammonium levels in chambers containing both a blacksmith and a young kelp plant were significantly lower than in chambers containing only a fish, and ammonium levels dropped in ammoniumspiked chambers that contained kelp plants. Nighttime ammonium uptake rates by young kelp plants, which averaged 1.6 μmol g-1 (dry) h-1, were only slightly lower than those during the day. Daytime excretion by blacksmiths occasionally results in elevated ammonium levels in the water column. On two of six days, ammonium concentrations in midwater foraging aggregations were slightly but significantly higher than in upcurrent controls; since blacksmiths typically aggregate at the incurrent margin of kelp beds, the ammonium is swept downcurrent and may be utilized by large M. pyrifera that extend through the water column. Thus, the activities of blacksmiths may results in the importation of extrinsic, inorganic nitrogen to primary producers on temperate reefs.

Patent
09 Oct 1986
TL;DR: In this article, a fiber optic sensor for sensing environmental effects on counterpropagating light beams in an optical loop by comparing the modulation of the light beams of an optical coil exposed to environmental effects and comparison with a reference fiber shielded from the environmental effects is provided.
Abstract: There is provided a fiber optic sensor for sensing environmental effects on counterpropagating light beams in an optical loop by comparing the modulation of the light beams in an optical coil exposed to the environmental effects and comparison with a reference fiber shielded from the environmental effects. The counterpropagating light paths contain optical phase modulators for creating nonreciprocal phase shifts and may comprise elongated sections forming a long line array.

Journal ArticleDOI
TL;DR: The early crystallization of plagioclase is consistent with the generally subophitic texture of the diabase as mentioned in this paper, which has a mildly alkaline, transitional character, and it is interpreted as a manifestation of widespread extensional tectonism.
Abstract: Diabase sills and dikes of Proterozoic age intrude crystalline basement and the overlying Crystal Spring Formation in the southern Death Valley region of California. Despite pervasive deuteric alteration, analyses of relict plagioclase (An66-45), titaniferous augite, and ilmenite permit the calculation of initial crystallization temperatures of 1,165±25° C for plagioclase and 1,110±25° C for augite with an oxygen fugacity of 10−11 atm. The early crystallization of plagioclase is consistent with the generally subophitic texture of the diabase. Geochemical arguments show that deuteric alteration has had little effect on the whole rock chemistry which has a mildly alkaline, transitional character. Mathematical models support the interpretation that chemical variations are principally the result of flow differentiation. Also, about 15 to 25% olivine or augite may have fractionated from the melt prior to intrusion. The mildly LREE enriched composition (normalized La/Yb=4.27 to 6.13) is best modelled by a 5% melt fraction from a moderately LREE-enriched source. Melt derived from a model garnet peridotite with accessory amphibole is compatible with the observed major and trace element chemistry. The diabase is compositionally akin to other middle Proterozoic basaltic rocks in North America which formed in extensional settings, and it is interpreted as a manifestation of widespread extensional tectonism.

Patent
23 Jun 1986
TL;DR: A feed supplement is a solidified mixture of various feed ingredients with a feed solution of sugars or proteins, and mixtures thereof, solidified by sodium carbonate, or a mixture thereof, and magnesium oxide.
Abstract: A buffer containing, solid, animal feed supplement which has the proper hardness and palatibility to achieve a daily animal consumption of 0.2-0.5 pound of sodium carbonate or bicarbonate and 0.07-0.15 pound of magnesium oxide with free choice feeding. The feed supplement is a solidified mixture of various feed ingredients with a feed solution of sugars or proteins, and mixtures thereof. The supplement is solidified by sodium carbonate, or a mixture thereof, and magnesium oxide.

Journal ArticleDOI
01 Dec 1986-Heredity
TL;DR: Developmental stability was assessed among lines of mice subjected to 11 generations of selection for increased (up line) and decreased (down line) widths of the first maxillary molar, primarily to test the hypothesis that this stability would decline over time as a result of selection.
Abstract: Developmental stability was assessed among lines of mice subjected to 11 generations of selection for increased (up line) and decreased (down line) widths of the first maxillary molar (M1), primarily to test the hypothesis that this stability would decline over time as a result of selection. Fluctuating asymmetry (FA) was used as an inverse measure of developmental stability, and was calculated for the M1 and a correlated character, the second mandibular molar (M2), in each generation. As measured by the regression of FA on generations, there was a statistically significant decline in stability only in the down selection line for the M2, and this trend did not differ significantly from that for the control line. The combined regressions obtained from pooling over all three lines were significantly different from zero in both molars, however, and this was taken to be evidence of a decline in stability due to increased homozygosity presumably generated by inbreeding during the selection experiment. The variation in FA among generations was greater for the selection lines than the control line for both molars, especially for the M2. The heritability of the M1 (and M2) was high, but there was no significant additive genetic variance for FA in either molar.

Journal ArticleDOI
TL;DR: The authors reviewed reasons why traditional faculty development programs have neglected middle-aged, disillusioned faculty (colleagues who are chronically depressed or angry about their jobs, who are inactive and unenthusiastic as teachers and researchers, and whose campus participation is passive at best) and described a format for engaging field-based developers, including chairpeople, in stepwise programs that help reestablish communication and reinvolvement of these "problem" faculty in meaningful activities and rewards.
Abstract: This paper reviews reasons why traditional faculty development programs have neglected middle-aged, disillusioned faculty (colleagues who are chronically depressed or angry about their jobs, who are inactive and unenthusiastic as teachers and researchers, and whose campus participation is passive at best). It then describes a format for engaging field-based developers, including chairpeople, in stepwise programs that help reestablish communication and reinvolvement of these “problem” faculty in meaningful activities and rewards. Data from ongoing field programs of the sort advocated here include survey-based portrayals of middle-aged, disillusioned faculty (e.g., 22% of all older faculty were rated as explosive with students/colleagues), of usual failings in communicating academic reward processes to faculty, of faculty's and administrators' reservations about reengaging “problem” colleagues, and of benefits accruing to field-workers themselves. Thus far, of the 45 middle-aged, disillusioned faculty involved in programs with their chairs acting as field-based developers, 84% have shown significant progress in preliminary stages of reestablishing communication and departmental activity, 69% have renegotiated for more meaningful roles as teachers/scholars/colleagues, and 47% have earned public rewards for meeting the goals set in their recontracting.

Patent
06 Nov 1986
TL;DR: A biologically stable animal food composition is described as having a crunchy shell of a low water activity consisting essentially of edible starch-and protein-containing flour at least partially surrounding a deformable core consisting of water soluble solids, which retains the distinct textures of the shell and core even after prolonged ambient exposure.
Abstract: A biologically stable animal food composition as described having a crunchy shell of a low water activity consisting essentially of edible starch- and protein-containing flour at least partially surrounding a deformable core of a low water activity including edible protein and water soluble solids, which composition retains the distinct textures of the shell and core even after prolonged ambient exposure.

Patent
11 Jun 1986
TL;DR: In this paper, a secure fiber optic communication system based on the Sagnac interferometer that allows multiple authorized users to communicate on the system by modulation and detection of two counter-propagating light beams is described.
Abstract: There is provided by this invention a secure fiber optic communication system based on the Sagnac interferometer that allows multiple authorized users to communicate on the system by modulation and detection of two counterpropagating light beams. The security of the system is based on a combination of the information being modulated on the relative phase difference between the counterpropagating light beams and the low coherence length of the light source. In order for an intruder to intercept the signal, both counterpropagating beams must be accessed and the pathlength of the two beams matched to within a few coherence lengths of the light source so that an amplitude modulated output signal may be constructed. The system is further comprised of a random pathlength generator used to randomly vary the relative pathlength of the two counterpropagating beams. A light level alarm is provided that detects changes in the light level within the system. If the signal level changes sufficiently, the light level alarm shuts down the system.

Journal ArticleDOI
TL;DR: It is suggested that verruciform xanthomas belong to a new category of "non-X histiocytoses" in which the presence of Langerhans cells suggests an immunologic pathogenesis.
Abstract: Ten oral verruciform xanthomas were studied using an immunoperoxidase stain for S-100 protein. All cases exhibited positively stained dendritic cells among the mononuclear inflammatory cell infiltrate at the base of the lesions and to a lesser extent among the "foam cells". The foam cells were, however, negative for S-100 staining. We suggest that, based on these findings, verruciform xanthomas belong to a new category of "non-X histiocytoses" in which the presence of Langerhans cells suggests an immunologic pathogenesis.

Journal ArticleDOI
TL;DR: Comparison with previous cases in the literature suggests that this particular deletion uniformly results in developmental delays, craniofacial changes, and occasionally results in microcephaly, low-set ears, and hand and foot abnormalities.
Abstract: We describe a malformed newborn girl with an interstitial deletion of the long arm of chromosome 2 (karyotype: 46, XX, del (2) (q31q33)). This is the first report of this particular chromosome abnormality that includes autopsy findings. Comparison with previous cases in the literature suggests that this particular deletion uniformly results in developmental delays, craniofacial changes, and occasionally results in microcephaly, low-set ears, and hand and foot abnormalities.

Proceedings ArticleDOI
01 Jan 1986
TL;DR: Los Angeles-Long Beach harbors were a grossly polluted body of water at the time of initiation of benthic invertebrate studies in 1951 as mentioned in this paper and a pollution abatement program was initiated in 1968 and has continued to this day.
Abstract: Los Angeles-Long Beach Harbors were a grossly polluted body of water at the time of initiation of benthic invertebrate studies in 1951. Waste discharges included industrial, domestic and storm waters which received little or no treatment. The inner harbor water mass contained little or no dissolved oxygen, but the outer harbor was well oxygenated. Benthic conditions were characterized by four different associations of animals plus an azoic zone. A pollution abatement program was initiated in 1968 and has continued to this day. Waste discharges were either eliminated or diverted to treatment plants. The effects of this abatement program were noted shortly thereafter and had a dramatic effect on the benthic fauna. The azoic and polluted zones were repopulated and are now characterized by a rich, varied benthic fauna. Benthic species known only previously from offshore areas are now resident inhabitants of the outer harbor. The usefulness of benthic monitoring over the past 35 years has demonstrated the rapidity of the improvement of ecological conditions as a result of pollution abatement. Furthermore, the knowledge gained from 35 years of study has provided a valuable data base as future changes in the harbor are contemplated.

Journal ArticleDOI
TL;DR: Ten abnormal hemoglobins were detected and characterized in individual cases referred to the authors' laboratory for evaluation of hematological problems and the mass spectra of the isolated abnormal peptide generally contained sufficient fragment ions to define the position of the amino acid substitution, obviating the need for lengthy sequencing procedures.
Abstract: Ten abnormal hemoglobins were detected and characterized in individual cases referred to our laboratory for evaluation of hematological problems. Six of these variants were electrophoretically sile...

Journal ArticleDOI
TL;DR: It is concluded that even if hyperbaric oxygen treatments are delayed 2 h, edema and muscle necrosis are reduced significantly in a model compartment syndrome.

Journal ArticleDOI
TL;DR: The authors compare the evolutionary logic of Civilization and Its Discontents with modern evolutionary logic, as represented by human sociobiology, and show that some of the theory used by modern sociobiologists to explain human behavior is formally similar to the arguments used by Freud.
Abstract: Recent scholarly work has emphasized the importance of evolutionary theory as an element of Freud's thought. This paper compares the evolutionary logic of Civilization and Its Discontents with modern evolutionary logic, as represented by human sociobiology. Some of Freud's arguments are quite modern, but although they were consistent with the evolutionary theory of his time, his arguments in many cases depart radically from a modern viewpoint: Moreover, a modern viewpoint would lead to the rejection of several of the psychic mechanisms proposed by Freud to explain human behavior. Nevertheless, the ultimate evolutionary questions that Freud posed are of continuing interest and must be answered by any theory of behavior. Moreover, some of the theory used by modern sociobiologists to explain human behavior is formally similar to the arguments used by Freud.

Patent
19 Feb 1986
TL;DR: In this article, a plurality of ultrasonic transducers mounted linearly on a belt that is contained and supported by a housing are used to test a test object along a line normal to the belt.
Abstract: A plurality of ultrasonic transducers mounted linearly on a belt that is contained and supported by a housing. The housing has wheels and handles mounted on its sides to allow an operator to grasp the apparatus and roll it across the surface of a test object along a line normal to the belt. The transducers are electrically connected to various devices which enable pulse echo testing to be performed on the test object and make the results immediately available as a picture either on paper or on a video monitor. The belt is moved laterally and cyclically back and forth as the apparatus is rolled longitudinally and the transducers are sequentially excited to emit ultrasonic waves, thus testing a swath having a width equal to the extremes of lateral motion of the line of transducers. A dynamic counterbalance swings 180° out of phase with the cyclic motion of the belt, and serves to damp out the vibration generated by the belt's motion. The transducers are held abutting the surface of the test object and oriented so that the emitted ultrasonic waves are always normally incident to this surface, regardless of whether the surface is curved or flat.


Journal ArticleDOI
TL;DR: It is concluded that although mortality after partial and total portal decompression is similar, the lower incidence of encephalopathy in survivors suggests that partial decompression has advantages over total decompression when emergency control of variceal bleeding is necessary.
Abstract: Emergency partial portal decompression was achieved with 8 or 10 mm portacaval H graft shunts combined with aggressive collateral ligation in 18 patients in whom bleeding esophageal varices could not be controlled medically. They were compared with 11 similar risk patients undergoing larger diameter portacaval H graft shunts (12 to 14 mm) for the same indications. Variables studied included 90 day operative mortality, hepatic encephalopathy rates, corrected portal pressure, and variceal rebleeding. Operative mortality was similar in both groups and correlated strongly with Child's class. However, the incidence of portasystemic encephalopathy in survivors was significantly lower after partial decompression than after total decompression. No patient in either group rebled from varices. We conclude from our series of high risk alcoholic cirrhotic patients, that although mortality after partial and total portal decompression is similar, the lower incidence of encephalopathy in survivors suggests that partial decompression has advantages over total decompression when emergency control of variceal bleeding is necessary.

Journal ArticleDOI
TL;DR: The procedures described were validated by confirming results obtained by other investigators using histology, immunocytochemistry, electron microscopy, and ISH.
Abstract: In order to obtain a useful and readily applicable in situ hybridization (ISH) protocol for progressive central nervous system (CNS) diseases of unknown etiology that are possibly due to persistent viral infection, known and well described diseases were studied, namely, progressive multifocal leukoencephalopathy (PML) and subacute sclerosing panencephalitis (SSPE). The procedures described were validated by confirming results obtained by other investigators using histology, immunocytochemistry, electron microscopy, and ISH. A number of frequently encountered problems of tissue preparation are addressed as well as techniques to reduce autoradiography exposure times. A multi-staged specific, sensitive, reliable, and valid procedure for detection of viral genomes, mRNA and proteins is approached. Formalin-fixed and paraffin-embedded (FFPE) brain material from six patients who died with PML and one patient who died from SSPE were studied using ISH with a tritium-labeled cloned JC virus DNA probe and a measles-cloned nucleocapsid (NC) gene cDNA probe, respectively. This report constitutes a methodological framework as well as a detailed neuropathological analysis of identified brain cell populations within which in situ hybridization was detected. In early PML lesions, swollen nuclei or oligodendrocytes were the predominant cells labeled, whereas older lesions revealed increased numbers of reactive and bizarre hypertrophic astrocytes hybridized at the outer periphery of the demyelinated lesions. The hybridization varied greatly in intensity in different cells. Intense hybridization was noted very rarely in microglial cells, including rod cells and rarely in venular pericytes, intravascular mononuclear cells, or in vascular endothelial cells. These results, considered together with previous findings, indicate that in PML the viral infection runs different courses in the various cells: in astrocytes the viral genome persists for a long time inducing pathological changes in some cells. In oligodendrocytes the infection rapidly lyses the cells. There was a good correlation between chromatic changes observable in routinely stained sections and virus presence. In addition, in situ hybridization using a measles-NP-cloned probe in white matter from FFPE SSPE brain is presented confirming earlier results in SSPE cryopreserved brain.

Journal ArticleDOI
TL;DR: The authors compare les valeurs calculees de la vitesse, des proprietes integrales et du cisaillement de Reynolds avec des donnees experimentales pour des ecoulements de sillage turbulents.
Abstract: On compare les valeurs calculees de la vitesse, des proprietes integrales et du cisaillement de Reynolds avec des donnees experimentales pour des ecoulements de sillage turbulents