Showing papers by "French Institute of Health and Medical Research published in 1972"
TL;DR: In chronic pancreatitis secondary to an obstruction in the main pancreatic duct, the lesions are different in almost every respect from those described above.
142 citations
TL;DR: It is conceivable that the hormone affects certain muscles through an increased (sexual or general) activity by way of the central nervous system, or because of the release of another hormone, or secondarily through some change of general metabolism.
Abstract: IT is not known whether the “anabolic” action of the androgenic hormone testosterone1, especially on muscular growth and metabolism, can be explained by the presence and the possible metabolism and “receptors” of the hormone in muscles. There are indeed several categories of muscles which, when dependent on androgens, may or may not respond directly to testosterone. It is conceivable that the hormone affects certain muscles through an increased (sexual or general) activity by way of the central nervous system, or because of the release of another hormone, or secondarily through some change of general metabolism.
139 citations
TL;DR: Since 1965, 359 parenteral injections of benzodiazepines were performed during polygraphic control in 220 epileptic children.
Abstract: SUMMARY
Since 1965, 359 parenteral injections of benzodiazepines were performed during polygraphic control in 220 epileptic children.
In 5 patients, who suffered with the syndrome of Lennox-Gastaut, i.v. injection of a benzodiazepine (diazepam or Nitrazepam®) was followed immediately by numerous tonic seizures, both subclinical and clinical, amounting to true tonic status.
All the patients with one exception were at the time of the injection in a state of confusion and showed numerous or continuous discharges of slow spike-waves.
All the patients had received other injections of benzodiazepine with little effect, and all except one had already had episodes of tonic status.
The brief delay between the injection and the appearance of seizures, the absence of clinical or EEG sleep at the time, and the temporal evolution of the tonic seizures after the injection of benzodiazepine: all these features exclude the possibility that the seizures were caused by sleep.
Resume
Depuis 1965, on a fait sous controle polygraphique, 359 injections parenterals, intra-veineuses pour la plupart, de benzodiazepines, chez des enfants epileptiques.
Dans 5 cas, classes comme syndromes de Lennox-Gastaut, une injection de benzodiazepine (diazepam ou Nitrazepam ®) a ete immediatement suivie de l'apparition de tres nombreuses crises toniques, infra-cliniques ou electrocliniques, realisant un veritable etat de mal tonique.
Tous les sujets (sauf un) etaient, au moment de l'injection, dans un etat d'obnubilation plus ou moins marque, avec des decharges de pointes-ondes lentes nombreuses,“ parfois meme subcontinues.
Tous les sujets avaient deja recu, a un moment ou a un autre, des injections de benzodiazepines avec peu d'effet, et tous avaient eu egalement (sauf un) des etats de mal et des crises toniques.
Le bref delai d'apparition des crises, l'absence de sommeil electroclinique et l'evolution dans le temps des crises toniques apres l'injection de diazepine, permettent d'exclure que les crises soient le resultat d'une simple activation par le sommeil.
127 citations
TL;DR: The implantation takes place at a moment when the concentration of receptor is very low, suggesting that implantation is controlled by progesterone secreted previously (perhaps in the pre-ovulatory period).
Abstract: The concentration of the progesterone cytosol receptor in the uterus shows marked variations during the estrus cycle. It is maximum in proestrus (about 40,000 binding sites per cell) and falls progressively during estrus and postestrus to attain a 16-fold lower value in diestrus. The receptor has a sedimentation coefficient (measured by sucrose gradient centrifugation) of 6.7 S during proestrus. In estrus, 6.7 S and 4.5 S binders are present, and the 4.5 S binder is predominant in postestrus and finally found exclusively in diestrus. One cannot differentiate 3 and 7 day pregnant animals by the concentration and the sedimentation coefficient of the progesterone receptors from the corresponding non-pregnant guinea pigs. Thus the implantation takes place at a moment when the concentration of receptor is very low, suggesting that implantation is controlled by progesterone secreted previously (perhaps in the pre-ovulatory period). The concentration of progesterone receptor in the uterus is positively controlle...
107 citations
TL;DR: The parietal and chief cell populations were measured in total gastrectomy specimens taken from 4 cases of the Zollinger-Ellison syndrome and there was a statistically significant correlation between the ratio of chief cell to parietal cell populations and the ratios of pepsin output to acid output.
102 citations
TL;DR: In chick liver nuclei obtained after centrifugation, there is a receptor of high affinity for estradiol, binding estrogens but not non-estrogenic hormones, and sensitive to proteolytic enzymes and p -chloromercuribenzoate.
99 citations
TL;DR: Purified liver plasma membranes isolated from adrenalectomized rats are characterized by a high sensitivity to glucagon and fluoride and an unusual sensitivity to epinephrine, thus allowing a comparative study of the two hormonal responses.
90 citations
TL;DR: The ability of phosphoproteins to increase RNA synthesis in the presence of nucleohistone is directly related to their phosphate content.
77 citations
TL;DR: It is now accepted that two classes of lymphocytes are present within the peripheral lymphoid tissues of the mammal: thymus-derived lymphocytes (T cells) and thymUS-independent, bone-marrow-derivedymphocytes (B cells).
Abstract: IT is now accepted that two classes of lymphocytes are present within the peripheral lymphoid tissues of the mammal: thymus-derived lymphocytes (T cells) and thymus-independent, bone-marrow-derived lymphocytes (B cells). So far much use has been made of isoantigenic markers on T cells to differentiate them from B cells1–5; markers which can be used on B cells are more recent6.
76 citations
TL;DR: The effects of intragastric and intravenous ethanol administration on exocrine secretion of human pancreas were studied and ethanol administration substantially reduced volume, concentration and output of bicarbonates, lipase and chymotrypsin.
Abstract: The effects of intragastric and intravenous ethanol administration on exocrine secretion of human pancreas were studied in 15 subjects. By either route, ethanol administration substantially reduced volume, concentration and output of bicarbonates, lipase and chymotrypsin. This action is interpreted as direct inhibition of the pancreas by ethanol. However, calcium concentration in duodenal juice increased with intragastric ethanol administration and decreased with intravenous ethanol administration.
73 citations
TL;DR: In this article, an improved procedure for automatic orthophosphate determination is described, which seems particularly suitable for a programme of standardisation, due to its accuracy, sensitivity, simplicity and high sample handling rate.
TL;DR: The proposed technique meets the necessary requirements for measuring receptor concentration in various physiological situations where endogenous progesterone varie...
Abstract: The concentration of progesterone receptor sites in uterine cytosol can be measured by microcolumn Sephadex G-25 chromatography under very strict experimental conditions. The ligand concentration must be kept in the 0.1–10 nM range and the protein concentration must be sufficiently low (=1 mg/ml) to minimize nonspecific binding. Under these conditions the saturation of the receptor is obtained and its apparent affinity for progesterone (K = 1.5 109 M1 at 0 C) and the binding sites concentration can be measured. When endogenous progesterone is present the technique can be used providing the time of incubation is shown to allow complete exchange between endogenous unlabelled and exogenous radioactive ligand. The apparent affinity for radioactive progesterone is then decreased but the concentration of the binding sites can still be measured. The proposed technique thus meets the necessary requirements for measuring receptor concentration in various physiological situations where endogenous progesterone varie...
TL;DR: In this paper, a sensitive automatic method for the determination of free or conjugated bile salts by means of 3α-hydroxysteroid dehydrogenase is described.
TL;DR: A large-scale purification of the soluble human placental oestradiol17-p dehydrogenase by affinity chromatography with an oestrone-3 linked Sepharose column is tried and the results of the amino acid analysis of this dehydrogen enzyme are reported.
TL;DR: A comparative study of the results obtained by the spectrophotometric method of Edelhoch25,27 and by the Chromatographie method of iodoamino acid analysis, it is concluded that the latter is more appropriate as an index of the iodoAMino acid composition of native thyroglobulin, especially when the iodine content of the protein is low.
TL;DR: The measurement of the rate of production and secretion of cortisol and cortisone by this method was shown to be impossible as evidenced by paradoxical results.
Abstract: Nine normal subjects (four men and five women), three patients with Cushing's syndrome, and three hyperthyroid females were given a constant infusion of 3H-cortisol and 14C-cortisone. In normal subjects the MCR of cortisone, 451 ± 79 (sd) L/m2/24 hr, was significantly greater than the MCR of cortisol, 155 ± 40 (sd) L/m2/24 hr. Both these clearances were increased in Cushing's syndrome and hyperthyroidism. In the normal subjects, the transfer constant measuring the conversion of cortisone into cortisol (ρ) 85 ± 8.5 (sd)% was significantly greater than that measuring the conversion of cortisol into cortisone (ρ) 60 ± 4.6 (sd)%, and was decreased in hyperthyroidism explaining the augmented urinary THE. The measurement of the rate of production and secretion of cortisol and cortisone by this method was shown to be impossible as evidenced by paradoxical results. The absence of a steady state in the production of cortisol did not appear to be the only cause of difficulty. The possibility that, when the...
TL;DR: It is suggested that PN and epileptic seizures can be phenomena of completely different natures even when they occur in epileptic subjects, and the possible finding of inter-ictal epileptiform abnormality in the EEGs of children with PN cannot be considered as proof of a link between epilepsy and PN.
TL;DR: The replacement of /3 92 (F8) histidine by the neutral residue glutamine is accompanied by absence of heme on the /3 chains, affords the first direct proof of the prediction of the Perutz model, that a covalent link is absolutely required in the binding of he me to globin.
TL;DR: It is concluded that native 19-S thyroglobulin contains polypeptides non-covalently associated with the bulk of the protein.
TL;DR: A new example of disturbance of this hydrogen bond is reported in this paper: in Hb Setif, the residue Asp Gl (94)01 is substituted by a tyrosine, which bears a genetic interest.
TL;DR: Histone fraction F2b isolated from Calf thymus, was fractionated by ion-exchange chromatography on Amberlite IRC-50 columns and an equimolecular complex of serine-rich, lysine- rich histone and of arginine-Rich, l Lysinerich histone was obtained.
TL;DR: Fractional phosphate excretion increased in the former and decreased in the latter, and clearance studies indicated a different behavior between fed and fasted rats undergoing saline diuresis.
TL;DR: The presence of colipase in the duodenal contents of a patient with congenital pancreatic lipase deficiency is demonstrated by immunological double-diffusion experiments in gel media.
TL;DR: Acidic and neutral amylo 1–4-glucosidase activities were clearly separated by cellulose acetate electrophoresis and an extra isozyme was found in kidney extracts.
TL;DR: The data suggest that the cortisone effect on muscular protein labeling may be independent of amino-acid penetration as reflected by AIB distribution, and the discrepant effects of the hormone in starved vs glucose loaded rats suggest that part of the cortisol response of the body is modulated by its nutritional state.
TL;DR: It appears that one of the earliest effects of oestradiol is to induce a “first-stage” protein synthesis, sufficiently slight to pass easily undetected by conventional techniques during the “lag period” of the first hour, but of crucial importance.
TL;DR: There are some indications that cytoplasmic dR NP may be derived from nuclear dRNP but no final conclusions can as yet be drawn because of uncertainty in the methods of analysis.