International Maize and Wheat Improvement Center

About: International Maize and Wheat Improvement Center is a nonprofit organization based out in Texcoco, Mexico. It is known for research contribution in the topics: Population & Agriculture. The organization has 1976 authors who have published 4799 publications receiving 218390 citations.

Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress

Abstract: Chickpea (Cicer arietinum L.) is an important grain-legume crop that is mainly grown in rainfed areas, where terminal drought is a major constraint to its productivity. We generated expressed sequence tags (ESTs) by suppression subtraction hybridization (SSH) to identify differentially expressed genes in drought-tolerant and -susceptible genotypes in chickpea. EST libraries were generated by SSH from root and shoot tissues of IC4958 (drought tolerant) and ICC 1882 (drought resistant) exposed to terminal drought conditions by the dry down method. SSH libraries were also constructed by using 2 sets of bulks prepared from the RNA of root tissues from selected recombinant inbred lines (RILs) (10 each) for the extreme high and low root biomass phenotype. A total of 3062 unigenes (638 contigs and 2424 singletons), 51.4% of which were novel in chickpea, were derived by cluster assembly and sequence alignment of 5949 ESTs. Only 2185 (71%) unigenes showed significant BLASTX similarity (<1E-06) in the NCBI non-redundant (nr) database. Gene ontology functional classification terms (BLASTX results and GO term), were retrieved for 2006 (92.0%) sequences, and 656 sequences were further annotated with 812 Enzyme Commission (EC) codes and were mapped to 108 different KEGG pathways. In addition, expression status of 830 unigenes in response to terminal drought stress was evaluated using macro-array (dot blots). The expression of few selected genes was validated by northern blotting and quantitative real-time PCR assay. Our study compares not only genes that are up- and down-regulated in a drought-tolerant genotype under terminal drought stress and a drought susceptible genotype but also between the bulks of the selected RILs exhibiting extreme phenotypes. More than 50% of the genes identified have been shown to be associated with drought stress in chickpea for the first time. This study not only serves as resource for marker discovery, but can provide a better insight into the selection of candidate genes (both up- and downregulated) associated with drought tolerance. These results can be used to identify suitable targets for manipulating the drought-tolerance trait in chickpea.

Assessment of the nitrogen management strategy using an optical sensor for irrigated wheat

Abstract: Blanket fertilizer nitrogen (N) recommendations for large irrigated wheat tracts lead to low N-use efficiency due to field-to-field variability in soil N supply and seasonal variability in yield. To achieve high N use efficiency, a site-specific N management strategy using GreenSeeker™ optical sensor was evaluated. We conducted seven field experiments during 2004–2006 at three locations to define relationships between in-season sensor measurements and yield of wheat and to know whether response of wheat to fertilizer N can be estimated by sensor measurements. During 2005–2007, four field experiments were conducted to assess the sensor-based N management strategy and to work out prescriptive N management to be followed prior to applying sensor-guided fertilizer dose. We observed robust relationships between in-season sensor-based estimates of yield at Feekes 5–6 and 7–8 stages and actual wheat yields. Response of wheat to fertilizer N defined by the sensor was highly correlated with harvest response index. Sensor-guided fertilizer N applications resulted in high yield levels and high N-use efficiency. Application of 90 kg N ha-1 at planting or in two equal doses at planting and crown root initiation stage was the appropriate prescriptive fertilizer N management. This study reveals that high N-use efficiency in irrigated wheat can be achieved by replacing blanket fertilizer recommendation by an optical sensor-based N management strategy consisting of applying moderate amount of fertilizer N at planting and crown root initiation stages and sensor-guided fertilizer N dose at Feekes 5–6 or 7–8 stages of wheat.

A global analysis of alternative tillage and crop establishment practices for economically and environmentally efficient rice production

Abstract: Alternative tillage and rice establishment options should aim at less water and labor to produce similar or improved yields compared with traditional puddled-transplanted rice cultivation. The relative performance of these practices in terms of yield, water input, and economics varies across rice-growing regions. A global meta and mixed model analysis was performed, using a dataset involving 323 on-station and 9 on-farm studies (a total of 3878 paired data), to evaluate the yield, water input, greenhouse gas emissions, and cost and net return with five major tillage/crop establishment options. Shifting from transplanting to direct-seeding was advantageous but the change from conventional to zero or reduced tillage reduced yields. Direct-seeded rice under wet tillage was the best alternative with yield advantages of 1.3–4.7% (p < 0.05) and higher net economic return of 13% (p < 0.05), accompanied by savings of water by 15% (p < 0.05) and a reduction in cost by 2.4–8.8%. Direct-seeding under zero tillage was another potential alternative with high savings in water input and cost of cultivation, with no yield penalty. The alternative practices reduced methane emissions but increased nitrous oxide emissions. Soil texture plays a key role in relative yield advantages, and therefore refinement of the practice to suit a specific agro-ecosystem is needed.

Identification of novel quantitative trait loci for days to ear emergence and flag leaf glaucousness in a bread wheat (Triticum aestivum L.) population adapted to southern Australian conditions

Abstract: In southern Australia, where the climate is predominantly Mediterranean, achieving the correct flowering time in bread wheat minimizes the impact of in-season cyclical and terminal drought. Flag leaf glaucousness has been hypothesized as an important component of drought tolerance but its value and genetic basis in locally adapted germplasm is unknown. From a cross between Kukri and RAC875, a doubled-haploid (DH) population was developed. A genetic linkage map consisting of 456 DArT and SSR markers was used to detect QTL affecting time to ear emergence and Zadoks growth score in seven field experiments. While ear emergence time was similar between the parents, there was significant transgressive segregation in the population. This was the result of segregation for the previously characterized Ppd-D1a and Ppd-B1 photoperiod responsive alleles. QTL of smaller effect were also detected on chromosomes 1A, 4A, 4B, 5A, 5B, 7A and 7B. A novel QTL for flag leaf glaucousness of large, repeatable effect was detected in six field experiments, on chromosome 3A (QW.aww-3A) and accounted for up to 52 percent of genetic variance for this trait. QW.aww-3A was validated under glasshouse conditions in a recombinant inbred line population from the same cross. The genetic basis of time to ear emergence in this population will aid breeders’ understanding of phenological adaptation to the local environment. Novel loci identified for flag leaf glaucousness and the wide phenotypic variation within the DH population offers considerable scope to investigate the impact and value of this trait for bread wheat production in southern Australia.

Trends in daily observed temperature and precipitation extremes over three Ethiopian eco-environments.

Abstract: Ethiopia has wide eco-environmental diversity ranging from extreme heat at one of the lowest places in the world to one of the coolest summits in Africa. Associated with this environmental diversity and climate change, climatic extremes are expected to change over time and also vary across eco-environments in the country. This study was conducted to examine the trends of past precipitation and temperature extremes over three eco-environments in Ethiopia. The study involved analysis of 20 extreme indices computed from daily temperature and precipitation data spanning over 42 years (1967–2008). The climate data were obtained from 11 stations selected from three major eco-environments (pastoral, agropastoral and highland). The results indicated positive trends for maximum value of the maximum temperature (TXx), warm days (TX90p), warm nights (TN90p) and warm spell duration indicators (WSDI) and negative trends for cool days (TX10p), cool nights (TN10p) and cold spell duration indicators (CSDI) in more than 8 of the 11 stations studied. However, most of the trends were not significant at many of the stations and the significant trends were not uniquely differentiated by eco-environments. Unlike temperature extremes, precipitation extreme trends showed high variability among nearby stations within eco-environments and were not significant at many of the stations studied. It is concluded that trends of temperature and precipitation extremes vary considerably among stations located within a given eco-environment indicating that the response of local climate to global warming could be different in physiographically diverse regions.