International Maize and Wheat Improvement Center

About: International Maize and Wheat Improvement Center is a nonprofit organization based out in Texcoco, Mexico. It is known for research contribution in the topics: Population & Agriculture. The organization has 1976 authors who have published 4799 publications receiving 218390 citations.

A study of allelic diversity underlying flowering-time adaptation in maize landraces

Abstract: Landraces (traditional varieties) of domesticated species preserve useful genetic variation, yet they remain untapped due to the genetic linkage between the few useful alleles and hundreds of undesirable alleles. We integrated two approaches to characterize the diversity of 4,471 maize landraces. First, we mapped genomic regions controlling latitudinal and altitudinal adaptation and identified 1,498 genes. Second, we used F-one association mapping (FOAM) to map the genes that control flowering time, across 22 environments, and identified 1,005 genes. In total, we found that 61.4% of the single-nucleotide polymorphisms (SNPs) associated with altitude were also associated with flowering time. More than half of the SNPs associated with altitude were within large structural variants (inversions, centromeres and pericentromeric regions). The combined mapping results indicate that although floral regulatory network genes contribute substantially to field variation, over 90% of the contributing genes probably have indirect effects. Our dual strategy can be used to harness the landrace diversity of plants and animals.

Genome sequencing of the high oil crop sesame provides insight into oil biosynthesis

Abstract: Background: Sesame, Sesamum indicum L., is considered the queen of oilseeds for its high oil content and quality, and is grown widely in tropical and subtropical areas as an important source of oil and protein. However, the molecular biology of sesame is largely unexplored. Results: Here, we report a high-quality genome sequence of sesame assembled de novo with a contig N50 of 52.2 kb and a scaffold N50 of 2.1 Mb, containing an estimated 27,148 genes. The results reveal novel, independent whole genome duplication and the absence of the Toll/interleukin-1 receptor domain in resistance genes. Candidate genes and oil biosynthetic pathways contributing to high oil content were discovered by comparative genomic and transcriptomic analyses. These revealed the expansion of type 1 lipid transfer genes by tandem duplication, the contraction of lipid degradation genes, and the differential expression of essential genes in the triacylglycerol biosynthesis pathway, particularly in the early stage of seed development. Resequencing data in 29 sesame accessions from 12 countries suggested that the high genetic diversity of lipid-related genes might be associated with the wide variation in oil content. Additionally, the results shed light on the pivotal stage of seed development, oil accumulation and potential key genes for sesamin production, an important pharmacological constituent of sesame. Conclusions: As an important species from the order Lamiales and a high oil crop, the sesame genome will facilitate future research on the evolution of eudicots, as well as the study of lipid biosynthesis and potential genetic improvement of sesame.

Association between Gene Lr34 for Leaf Rust Resistance and Leaf Tip Necrosis in Wheat

Abstract: Durable resistance to leaf rust (Puceinia recondita Roberge ex Desmaz. f. sp. tritici) in bread wheat (Triticum oestivurn L.) cultivars is known to result from the interaction of Lr34 with other minor additive genes that are effective in the adult growth state. The Lr34 gene seems to be present in several CIMMYT germplasm-derived Mexican cultivars that display temperature- and light-sensitive seedling responses similar to those displayed by near-isogenic ‘Thatcher’ lines that contain Lr34. Adult plants of these two Thatcher lines and all Mexican cultivars postulated to carry Lr34 display resistance to leaf rust and exhibit leaf tip necrosis symptoms. This study was conducted to determine whether 15-34 and a gene or genes for leaf tip necrosis am linked. The simultaneous presence of Lr34 and leaf tip necrosis in the two Thatcher near-isogenic lines and Mexican cultivars was confirmed by evaluating F₂ plants or F₃ lines obtained from various intercrosses. Lr34 and leaf tip necrosis were inherited together in a population of 117 F₃ lines obtained from the cross of leaf rust resistant ‘Jupateco 73R’ (carrying Lr34 and leaf tip necrosis) with its susceptible counterpart ‘Jupateco 73s’. Linkage or pleiotropism was also evident in the crosses of six Lr34-carrying Mexican cultivars with ‘Siete Cerros 66’ (which does not have Lr34). The gene for leaf tip necrosis, designated Lln, could be used as a marker for Lr34.

Influence of permanent raised bed planting and residue management on physical and chemical soil quality in rain fed maize/wheat systems

Abstract: Densely populated, intensively cropped highland areas in the tropics and subtropics are prone to erosion and declining soil fertility, making agriculture unsustainable. Conservation agriculture in its version of permanent raised bed planting with crop residue retention has been proposed as an alternative wheat production system for this agro-ecological zone. A five years field experiment comparing permanent and tilled raised beds with different residue management under rainfed conditions was started at El Batan (Mexico) (2,240 m asl; 19.31°N, 98.50°W; Cumulic Phaeozem) in 1999. The objective of this study was to determine the soil quality status after five years of different management practices. The K concentration was 1.65 times and 1.43 times larger in the 0–5 cm and 5–20 cm profiles, respectively, for permanent raised beds compared to conventionally tilled raised beds. The Na concentration showed the opposite trend. Sodicity was highest for conventionally tilled raised beds and for permanent raised beds it increased with decreasing amounts of residue retained on the surface. Permanent raised beds with full residue retention increased soil organic matter content 1.4 times in the 0–5 cm layer compared to conventionally tilled raised beds with straw incorporated and it increased significantly with increasing amounts of residue retained on the soil surface for permanent raised beds. Soil from permanent raised beds with full residue retention had significantly higher mean weight diameter for wet and dry sieving compared to conventionally tilled raised beds. Permanent raised beds with full residue retention had significantly higher aggregate stability compared to those with residue removal. A lower aggregation resulted in a reduction of infiltration. Principal component analysis (PCA) was performed using these soil physicochemical variables that were significantly influenced by tillage or residue management. The PC1 and PC2 separated the conventionally tilled raised beds from the permanent raised beds and PC3 separated permanent raised beds with at least partial residue retention from permanent raised beds with no residue retention. These clear separations suggest that tillage and residue management have an effect on soil processes. The research indicates that permanent raised bed planting increases the soil quality and can be a sustainable production alternative for the (sub)tropical highlands. Extensive tillage with its associated high costs can be reduced by the use of permanent raised beds while at least partial surface residue retention is needed to insure production sustainability.