International Maize and Wheat Improvement Center

About: International Maize and Wheat Improvement Center is a nonprofit organization based out in Texcoco, Mexico. It is known for research contribution in the topics: Population & Agriculture. The organization has 1976 authors who have published 4799 publications receiving 218390 citations.

Molecular detection of genomic regions associated with grain yield and yield-related components in an elite bread wheat cross evaluated under irrigated and rainfed conditions

Abstract: Grain yield and grain weight of wheat are often decreased by water-limitation in the north-eastern cropping belt of Australia. Based on knowledge that CIMMYT lines are well-adapted in this region, a recombinant inbred line (RIL) population between two elite CIMMYT bread wheats (Seri M82 and Babax) was evaluated under water-limited environments. Fourteen productivity traits were evaluated in 192 progeny in up to eight trials. For three aggregations of the environments (all, high yield or low yield), multiple quantitative trait loci (QTL) were detected, each explaining 3) from Seri (6D-b and UA-d) increased grain yield and co-located with a suggestive (2 4t ha−1) environments. A third increased grain yield QTL (7A-a) from Babax co-located with QTL for increased grain number. Six putative QTL increased grain weight and co-located with QTL for harvest index, grains per spike and spike number. Three putative QTL for increased grains per spike co-located with strong QTL for earlier flowering, increased grain weight and fewer spikes. A group of progeny that exceeded the mean grain yield and grain weight of commercial checks had an increased frequency of QTL for high WSC, large grain size, increased harvest index and greater height, but fewer stems, when compared to low yielding (20% less), low grain weight progeny. These findings were consistent with agronomic analyses of the germplasm and demonstrate that there should be opportunities to independently manipulate grain number and grain size which is typically difficult due to strong negative correlations.

Nucleotide diversity maps reveal variation in diversity among wheat genomes and chromosomes

Abstract: A genome-wide assessment of nucleotide diversity in a polyploid species must minimize the inclusion of homoeologous sequences into diversity estimates and reliably allocate individual haplotypes into their respective genomes. The same requirements complicate the development and deployment of single nucleotide polymorphism (SNP) markers in polyploid species. We report here a strategy that satisfies these requirements and deploy it in the sequencing of genes in cultivated hexaploid wheat (Triticum aestivum, genomes AABBDD) and wild tetraploid wheat (Triticum turgidum ssp. dicoccoides, genomes AABB) from the putative site of wheat domestication in Turkey. Data are used to assess the distribution of diversity among and within wheat genomes and to develop a panel of SNP markers for polyploid wheat. Nucleotide diversity was estimated in 2114 wheat genes and was similar between the A and B genomes and reduced in the D genome. Within a genome, diversity was diminished on some chromosomes. Low diversity was always accompanied by an excess of rare alleles. A total of 5,471 SNPs was discovered in 1791 wheat genes. Totals of 1,271, 1,218, and 2,203 SNPs were discovered in 488, 463, and 641 genes of wheat putative diploid ancestors, T. urartu, Aegilops speltoides, and Ae. tauschii, respectively. A public database containing genome-specific primers, SNPs, and other information was constructed. A total of 987 genes with nucleotide diversity estimated in one or more of the wheat genomes was placed on an Ae. tauschii genetic map, and the map was superimposed on wheat deletion-bin maps. The agreement between the maps was assessed. In a young polyploid, exemplified by T. aestivum, ancestral species are the primary source of genetic diversity. Low effective recombination due to self-pollination and a genetic mechanism precluding homoeologous chromosome pairing during polyploid meiosis can lead to the loss of diversity from large chromosomal regions. The net effect of these factors in T. aestivum is large variation in diversity among genomes and chromosomes, which impacts the development of SNP markers and their practical utility. Accumulation of new mutations in older polyploid species, such as wild emmer, results in increased diversity and its more uniform distribution across the genome.

A Direct Comparison of Remote Sensing Approaches for High-Throughput Phenotyping in Plant Breeding

Abstract: Remote sensing (RS) of plant canopies permits non-intrusive, high-throughput monitoring of plant physiological characteristics. This study compared three RS approaches using a low flying UAV (unmanned aerial vehicle), with that of proximal sensing, and satellite-based imagery. Two physiological traits were considered, canopy temperature (CT) and a vegetation index (NDVI), to determine the most viable approaches for large scale crop genetic improvement. The UAV-based platform achieves plot-level resolution while measuring several hundred plots in one mission via high-resolution thermal and multispectral imagery measured at altitudes of 30-100 m. The satellite measures multispectral imagery from an altitude of 770 km. Information was compared with proximal measurements using IR thermometers and an NDVI sensor at a distance of 0.5-1 m above plots. For robust comparisons, CT and NDVI were assessed on panels of elite cultivars under irrigated and drought conditions, in different thermal regimes, and on un-adapted genetic resources under water deficit. Correlations between airborne data and yield/biomass at maturity were generally higher than equivalent proximal correlations. NDVI was derived from high-resolution satellite imagery for only larger sized plots (8.5 × 2.4 m) due to restricted pixel density. Results support use of UAV-based RS techniques for high-throughput phenotyping for both precision and efficiency.

Resequencing of 429 chickpea accessions from 45 countries provides insights into genome diversity, domestication and agronomic traits.

Abstract: We report a map of 4.97 million single-nucleotide polymorphisms of the chickpea from whole-genome resequencing of 429 lines sampled from 45 countries. We identified 122 candidate regions with 204 genes under selection during chickpea breeding. Our data suggest the Eastern Mediterranean as the primary center of origin and migration route of chickpea from the Mediterranean/Fertile Crescent to Central Asia, and probably in parallel from Central Asia to East Africa (Ethiopia) and South Asia (India). Genome-wide association studies identified 262 markers and several candidate genes for 13 traits. Our study establishes a foundation for large-scale characterization of germplasm and population genomics, and a resource for trait dissection, accelerating genetic gains in future chickpea breeding.

Evaluating physiological traits to complement empirical selection for wheat in warm environments

Abstract: The response of spring wheat to heat stress has been determined in several hot wheat growing environments worldwide on different types of germplasm. Physiological data has been collected to identify potential traits to assist in the empirical breeding for heat tolerance. Initial studies focused on 10 established varieties to determine genetic diversity for heat tolerance, identify association between heat tolerance and traits measured, and evaluate genotype by environment interaction (G × E). Yields from over 40 hot environments were analysed for G x E, and relative humidity (RH) was identified as the major factor determining relative genotype ranking. Further analysis focused on 16 environments: those with low RH and relatively high yields, i.e., over 2.5 t ha−1. For these environments, mean yield of lines correlated with a number of physiological traits measured in Mexico, including canopy temperature depression (CTD), membrane thermostability, leaf conductance and photosynthetic rate at heading, chlorophyll content during grainfilling, leaf internal CO2 concentration, and dark respiration. Morphological traits were measured in all environments and the following showed associations with yield: above ground biomass at maturity, days from emergence to anthesis and to maturity, grain number m−2, and ground cover estimated visually after heading. Subsequent studies focused on breeding material, namely recombinant inbred lines derived from crosses between parents of contrasting heat tolerance, and 60 advanced breeding lines selected for performance under heat stress. The genetic basis for association between heat tolerance and CTD was established by demonstrating a correlation between the two traits in RILs (recombinant inbred lines). Data from RILs, as well as from the 60 advanced lines grown at several international locations, indicated CTD to be a powerful and robust selection criterion for heat tolerance.