International Maize and Wheat Improvement Center

About: International Maize and Wheat Improvement Center is a nonprofit organization based out in Texcoco, Mexico. It is known for research contribution in the topics: Population & Agriculture. The organization has 1976 authors who have published 4799 publications receiving 218390 citations.

QTL Mapping in Tropical Maize: II. Comparison of Genomic Regions for Resistance to Diatraea spp.

Abstract: Southwestern corn borer (SWCB), Diatraea grandiosella (Dyar), and the sugar cane borer (SCB), Diatraea saccharalis (Fabricius), are serious insect pests in maize (Zea mays L.) production areas of Central America and the southern USA. We mapped and characterized quantitative trait loci (QTL) affecting resistance to the leaf feeding generation of SWCB(1 SWCB), compared these QTL with those for resistance to the leaf feeding generation of SCB (1SCB) identified in the same mapping population, and assessed the consistency of QTL for 1SWCB across two populations. One hundred seventy-one F2 genotypes from cross CML131 (susceptible) × CML67 (resistant) and 100 RFLP marker loci were used for the QTL analyses. 1SWCB and 1SCB resistance were assessed in F2:3 lines by leaf damage ratings (LDR) after artificial infestation in field experiments with two replications at one subtropical environment in 2 yr. The method of composite interval mapping (CIM) was used for QTL detection. Estimates of genotypic (σ2g) and genotype × year interaction variance (σ2g) were highly significant for 1SWCB LDR and 1SCB LDR. Phenotypic and genotypic correlations between both traits were 0.62 and 1.02, respectively. for 1SWCB LDR, six QTL were detected explaining 53.3% of σ2g, with two QTL displaying significant QTL × year interactions. Ten QTL were detected for 1SCB LDR, accounting for 98.2% of σ2g. The QTL showed predominantly additive or partially dominant gene action. Seven out of 10 QTL were pleiotropic to both Diatraea spp. Three genomic regions, on Chromosomes 5 and 9, were consistent with a second mapping population derived from cross Ki3 (susceptible) × CML139 (resistant), for which seven QTL for 1SWCB LDR were found. Marker-assisted ‘gene stacking’ is recommended for transferring pleiotropic QTL into susceptible germplasm and for pyramiding QTL from different sources of insect resistance.

Impact of adoption of drought-tolerant maize varieties on total maize production in south Eastern Zimbabwe

Abstract: Drought is a huge limiting factor in maize production, mainly in the rain-fed agriculture of sub-Saharan Africa. In response to this threat, drought-tolerant (DT) maize varieties have been developed with an aim to ensure maize production under mild drought conditions. We conducted a study to assess the impact of smallholder farmers’ adoption of DT maize varieties on total maize production. Data for the study came from a survey of 200 randomly sampled households in two districts of Chiredzi and Chipinge in southeastern Zimbabwe. The study found that 93% of the households were growing improved maize varieties and that 30% of the sampled households were growing DT maize varieties. Total maize yield was 436.5 kg/ha for a household that did not grow DT maize varieties and 680.5 kg/ha for households that grew DT maize varieties. We control for the endogeneity of the DT adoption variable, by using the control function approach to estimate total maize production in a Cobb–Douglas model. The results show that households that grew DT maize varieties had 617 kg/ha more maize than households that did not grow the DT maize varieties. Given that almost all farmers buy their seeds in the market, a change in varieties to DT maize seeds gives an extra income of US$240/ha or more than nine months of food at no additional cost. This has huge implications in curbing food insecurity and simultaneously saving huge amounts of resources at the household and national levels, which are used to buy extra food during the lean season.

Development of a maize 55 K SNP array with improved genome coverage for molecular breeding

Abstract: With the decrease of cost in genotyping, single nucleotide polymorphisms (SNPs) have gained wide acceptance because of their abundance, even distribution throughout the maize (Zea mays L.) genome, and suitability for high-throughput analysis. In this study, a maize 55 K SNP array with improved genome coverage for molecular breeding was developed on an Affymetrix® Axiom® platform with 55,229 SNPs evenly distributed across the genome, including 22,278 exonic and 19,425 intronic SNPs. This array contains 451 markers that are associated with 368 known genes and two traits of agronomic importance (drought tolerance and kernel oil biosynthesis), 4067 markers that are not covered by the current reference genome, 734 markers that are differentiated significantly between heterotic groups, and 132 markers that are tags for important transgenic events. To evaluate the performance of 55 K array, we genotyped 593 inbred lines with diverse genetic backgrounds. Compared with the widely-used Illumina® MaizeSNP50 BeadChip, our 55 K array has lower missing and heterozygous rates and more SNPs with lower minor allele frequency (MAF) in tropical maize, facilitating in-depth dissection of rare but possibly valuable variation in tropical germplasm resources. Population structure and genetic diversity analysis revealed that this 55 K array is also quite efficient in resolving heterotic groups and performing fine fingerprinting of germplasm. Therefore, this maize 55 K SNP array is a potentially powerful tool for germplasm evaluation (including germplasm fingerprinting, genetic diversity analysis, and heterotic grouping), marker-assisted breeding, and primary quantitative trait loci (QTL) mapping and genome-wide association study (GWAS) for both tropical and temperate maize.

A gene for resistance to the maize streak virus in the African CIMMYT maize inbred line CML202

Abstract: Resistance to maize streak virus (MSV) is an essential trait of improved maize varieties in sub-Saharan Africa. We mapped quantitative trait loci (QTL) for resistance to MSV in a population of 196 F2:3 lines derived from a cross between the maize inbred lines CML202 (resistant) from CIMMYT-Zimbabwe and Lo951 (susceptible) from Italy. Field tests were planted at two locations in Zimbabwe, inoculated with viruliferous leaf hoppers (Cicadulina mbila), and scored twice (21 and 83 days after infesting, DAI) on a 1–5 scale. The mean final streak intensity (score 2) of the parent lines was 2.2 (CML202) and 4.8 (Lo951). Genotype × location interaction was large for score 1 but negligible for score 2. Consequently, the heritability was higher for score 2 (0.93) than for score 1 (0.62). By composite interval mapping across locations, using a linkage map with 110 RFLP loci, four significant (LOD ≥3.0) QTL were identified for score 1 on chromosomes (C) 1, 2, 3, and 4, respectively. All four were contributed by CML202. For score 2, only the QTL on C 1 was significant (LOD =37), explaining 59% of the phenotypic and 64% of the genotypic variance. The QTL's partially dominant gene action was consistent with the nearly intermediate resistance of the F1 generation (relative heterosis for resistance 12%). The presence of one major QTL is consistent with the bimodal frequency distribution of the mapping population showing a clear 3:1 segregation. This gene seems to be allelic or identical to Msv1, a major resistance gene which was previously identified in the same genomic region in Tzi4, an inbred line from IITA. Inbred CML202 had lower final disease ratings than Tzi4. The greater resistance of CML202 may be due to allelic differences at the msv1 locus or due to the minor QTL on C 2, 3, and 4 which were not detected in Tzi4.

Complementary practices supporting conservation agriculture in southern Africa: A review

Abstract: Conservation agriculture (CA)—the simultaneous application of minimum soil disturbance, crop residue retention, and crop diversification—is a key approach to address declining soil fertility and the adverse effects of climate change in southern Africa. Applying the three defining principles of CA alone, however, is often not enough, and complementary practices and enablers are required to make CA systems more functional for smallholder farmers in the short and longer term. Here, we review 11 complementary practices and enablers grouped under six topical areas to highlight their critical need for functional CA systems, namely: (1) appropriate nutrient management to increase productivity and biomass; (2) improved stress-tolerant varieties to overcome biotic and abiotic stresses; (3) judicious use of crop chemicals to surmount pest, diseases, and weed pressure; (4) enhanced groundcover with alternative organic resources or diversification with green manures and agroforestry; (5) increased efficiency of planting and mechanization to reduce labor, facilitate timely planting, and to provide farm power for seeding; and (6) an enabling political environment and more harmonized and innovative extension approaches to streamline and foster CA promotional efforts. We found that (1) all 11 complementary practices and enablers substantially enhance the functioning of CA systems and some (e.g., appropriate nutrient management) are critically needed to close yield gaps; (2) practices and enablers must be tailored to the local farmer contexts; and (3) CA systems should either be implemented in a sequential approach, or initially at a small scale and grow from there, in order to increase feasibility for smallholder farmers. This review provides a comprehensive overview of practices and enablers that are required to improve the productivity, profitability, and feasibility of CA systems. Addressing these in southern Africa is expected to stimulate the adoption of CA by smallholders, with positive outcomes for soil health and resilience to climate change.